山绿茶总黄酮对前列腺增生大鼠细胞增殖的影响  

Effect of Hainan holly leaf total flavonoids on proliferation of prostate cells in rats with benign prostatic hyperplasia

作　　者：盛树东[1] 于有江[1] 王冬艳[1] 钱小妹[1] 肖望书[1] 

机构地区：[1]扬州环境资源职业技术学院医学系,江苏省225127

出　　处：《江苏医药》2012年第8期875-877,共3页Jiangsu Medical Journal

基　　金：江苏省卫生厅资助项目(J201009)

摘　　要：目的观察前列腺增生大鼠前列腺组织增殖细胞核抗原(PCNA)和Ki-67的表达及山绿茶总黄酮(HHLTF)对其影响。方法 48只SD大鼠随机均分为前列腺增生模型(P)组、前列康治疗(Q)组、HHLTF高(H1)、中(H2)、低(H3)剂量治疗组和对照(C)组。除C组外各组大鼠去势后注射丙酸睾酮制作前列腺增生模型,用前列康(570mg.kg-1.d-1)或HHLTF 80、40、20mg.kg-1.d-1灌胃,连续5周;P、C组以生理盐水灌胃。计算大鼠前列腺指数,免疫组化法测定PCNA和Ki-67的表达。结果 H1、H2组的前列腺指数及PCNA、Ki-67表达均较P组显著降低(P<0.01)。结论HHLTF具有抑制前列腺增生的作用,其机制可能是通过抑制PCNA和Ki-67蛋白的表达而实现。Objective To observe the effect of Hainan holly leaf total flavonoids（HHLTF） on the expressions of proliferating cell nuclear antigen （PCNA） and Ki-67 in rats with prostatic hyperplasia（PH）. Methods Forty-eight male SD rats were equally randomized into six groups of P （PH model＋gastric gavage with normal saline）, Q（PH model＋gastric gavage with Qianliekang 570 mg· kg^-1· d^-1 ）, H1, H2, H3 （PH model ＋ gastric gavage with HHLTF 80,40, 20 mg · kg^-1·d^-1 , respectively） and C（gastric gavage with normal saline as blank control）. The PH model was made by subcutaneous injection of testosterone propionate after surgical castration and treatment lasted for 5 weeks, after which the index of prostate gland（PI） was calculated and the expressions of PCNA and Ki-67 in prostate tissues were detected by immunohistochemistry. Results PI and the expressions of PCNA and Ki-67 in prostate tissues were significantly lower in groups of HI and H2 than those in group P（P（0. 01）. Conclusion HHLTF can inhibit proliferation of the prostate gland,which may be through inhibiting the expressions of PCNA and Ki-67.

关 键 词：山绿茶总黄酮 前列腺增生 增殖细胞核抗原 KI-67 

分 类 号：R285[医药卫生—中药学]