蛹虫草(北冬虫夏草)提取物对高糖诱导的内皮细胞衰老和细胞内活性氧的影响  

Influence of extract of Cordyceps militaris on senescence of endothelial cells and reactive oxygen species in cells induced by high glucose

作　　者：叶章正[1] 朱鹏立[1] 余惠珍[1] 黄华玮[1] 阮景明[1] 林帆[1] 

机构地区：[1]福建医科大学省立临床医学院,福建省立医院干部特诊科,福建省临床老年病研究所

出　　处：《心血管康复医学杂志》2012年第2期129-132,共4页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：福建省医学创新课题(2011-CXB-3)

摘　　要：目的:探讨蛹虫草乙酸乙酯提取物(GSCME)对高糖诱导人脐静脉内皮细胞(HUVECs)的衰老和细胞内活性氧的影响。方法:体外培养HUVECs,分3组:对照组(D-葡萄糖5.5mmol/L)、高糖组(D-葡萄糖40mmol/L)、GSCME组(GSCME 50μg/ml+D-葡萄糖40mmol/L),检测各组细胞增殖情况,SA-β-gal阳性衰老细胞,周期分布和细胞内活性氧(ROS)含量。结果:与对照组相比,高糖干预24、48、72h后细胞增殖活性显著下降[OD:48h:(0.599±0.062)比(0.375±0.013)],P均<0.01;高糖孵育48h后SA-β-gal阳性细胞率[(50.70±1.49)%比(89.63±0.69)%]、G0/G1期细胞百分率[(49.15±2.56)%比(76.59±3.58)%]、细胞内活性氧(ROS)水平[(1.03±0.15)比(3.60±0.36)]均明显增加,P均<0.01。与高糖组相比,GSCME孵育48h、72hHUVECs增殖率显著上升[48h:(0.375±0.013)比(0.424±0.023)],P均<0.05,孵育48h显著降低SA-β-gal阳性细胞率[(89.63±0.69)%比(65.81±1.50)%]、G0/G1期细胞百分率[(76.59±3.58)%比(52.70±5.64)%]及细胞内ROS水平[(3.60±0.36)比(1.67±0.21)],P<0.05～0.01。结论:蛹虫草提取物可延缓高糖诱导的人脐静脉内皮细胞衰老进程,降低细胞内活性氧从而减轻细胞氧化损伤可能是其作用的机制之一。Objective： To study influence of ethyl acetate extract of Cordyceps militaris（GSCME） on senescence of human umbilical vein endothelial cells（HUVECs） and reactive oxygen species（ROS） in cells induced by high glucose.Methods：HUVECs were cultured in vitro and divided into control group（cultured with D-glucose 5.5 mmol/L）,high glucose group（cultured with D-glucose 40 mmol/L） and GSCME group（cultured with GSCME 50 μg/ml＋D-glucose 40 mmol/L）.HUVECs proliferation,SA-β-gal positive senescence cell,distribution of cell cycles and concentrations of ROS in cells were measured in each group.Results：Compared with control group,after incubated with high glucose for 24,48 and 72h,proliferation activity of HUVECs significantly decreased,P0.05 all];after incubated with high glucose for 48h,there were significant increase in SA-β-gal positive cell rate [（50.70±1.49）% vs.（89.63±0.69）%],percentage of G0/G1 period cells [（49.15±2.56）% vs.（76.59±3.58）%]and level of ROS [（1.03±0.15） vs.（3.60±0.36）],P0.01 all.Compared with high glucose group,HUVECs proliferation rate significantly increased after incubated with GSCME for 48h and 72h [48h：（0.375±0.013） vs.（0.424±0.023）,]P0.05 both;after incubation with CME for 48h,there were significant decrease in SA-β-gal positive cell rate [（89.63±0.69）% vs.（65.81±1.50）%],percentage of G0/G1 period cells [（76.59±3.58）% vs.（52.70±5.64）%]and ROS level in cells [（3.60±0.36） vs.（1.67±0.21） ]in GSCME group,P0.05~0.01.Conclusion： GSCME can delay senescence progression of HUVECs induced by high glucose.One of its possible mechanisms may be ROS level in cell decrease,so it decreases oxidative damage of cells.

关 键 词：内皮细胞 细胞衰老 抗氧化剂 

分 类 号：R977.9[医药卫生—药品]