缺血后处理对高血脂大鼠缺血/再灌注心肌Caspase-3活性的影响  

Influence of ischemic postconditioning on Caspase-3 activity in myocardium of rats with hyperlipidemia after ischemia/reperfusion

作　　者：安莉[1] 霍玉娥[1] 刘胜辉[1] 

机构地区：[1]河北大学附属医院心内科,河北保定市071000

出　　处：《心血管康复医学杂志》2012年第2期175-177,共3页Chinese Journal of Cardiovascular Rehabilitation Medicine

摘　　要：目的:观察缺血后处理对高血脂大鼠缺血再灌注心肌Caspase(半胱氨酸天冬氨酸酶)-3活性的影响。方法:选择高血脂SD大鼠48只,随机分为3组:假手术组(开胸后穿线做套环,但不收紧结扎线)、缺血再灌注组(收紧结扎线缺血40min,放松结扎线再灌注240min)、缺血后处理组(缺血40min后,再灌注10s,缺血10s,连续3个循环,然后再灌注240min),每组16只。再灌注结束后自右颈动脉采血,测定血清肌酸激酶(CK)活性,再灌注心肌凋亡程度,及Caspase-3活性,并进行比较分析。结果:再灌注结束后,①缺血后处理组和缺血再灌注组CK活性明显高于假手术组[(745.26±62.18)U/L比(926.38±76.49)U/L比(237.67±21.82)U/L],缺血后处理组明显低于缺血再灌注组(P均<0.05);②心肌凋亡细胞计数:假手术组未见明显细胞凋亡(<5%),缺血后处理组心肌细胞凋亡率明显低于缺血再灌注组[(11.7±2.6)%比(20.8±3.4)%,P<0.05];③缺血后处理组缺血区心肌组织Caspase-3活性明显低于缺血再灌注组[(545.79±98.25)μmol PNA/mg比(739.83±113.57)μmol PNA/mg,P<0.01]。结论:缺血后处理可以减轻高血脂大鼠缺血再灌注损伤,其心肌保护作用可能与降低Caspase-3活性有关。Objective： To study influence of ischemic postconditioning on Caspase（cysteine aspartase）-3 activity in myocardium of rats with hyperlipidemia after ischemia/reperfusion（I/R）.Methods：A total of 48 SD rats with hyperlipidemia were randomly divided into sham operation group（n=16）,I/R group（n=16,ligated to cause ischemia for 40 min,then reperfused for 240 min） and ischemic postconditioning group（n=16,after 40 min ischemia,reperfused for 10s and ischemia for 10s for three cycles,then reperfused for 240 min）.After reperfusion,blood sample was taken from right carotid artery to measure activity of creatine kinase（CK）,Caspase-3 activity and apoptotic extent of myocardial cells.These indicators were compared and analyzed among three groups.Results：After reperfusion,① CK activity in ischemic postconditioning group and I/R group were significantly higher than that of sham operation group [（745.26±62.18） U/L vs.（926.38±76.49） U/L vs.（237.67±21.82） U/L],and that of ischemic postconditioning group was significantly lower than that of I/R group（P0.05 all）;② There was no obvious apoptosis（5%） in sham operation group,apoptosis rate of myocardial cells in ischemic postconditioning group was significantly lower than that of I/R group [（11.7±2.6）% vs.（20.8±3.4）%,P0.05];③ Caspase-3 activity in ischemic myocardial tissues in ischemic postconditioning group was significantly lower than that of I/R group [（545.79±98.25） μmol PNA/mg vs.（739.83±113.57） μmol PNA/mg,P0.01].Conclusion： Ischemic postconditioning can relieve ischemia/reperfusion injury in rats with hyperlipidemia;its myocardial protection may be related with decrease of Caspase-3.

关 键 词：高脂血症 再灌注损伤 心肌 细胞凋亡 

分 类 号：R589.2[医药卫生—内分泌]