五味子提取物预防辐射所致免疫损伤的实验研究  

Schisandra Chinensis extract on immune injury by radiation

作　　者：刘丽华[1,2] 刘登湘[2,3] 马鸣[2] 张璁[2] 段玉青[1] 赵连梅[2] 单保恩[2] 

机构地区：[1]河北医科大学第四医院临床生物细胞室,河北石家庄050011 [2]河北医科大学第四医院科研中心,河北石家庄050011 [3]邢台市人民医院放疗科,河北邢台054000

出　　处：《癌变．畸变．突变》2012年第2期108-110,115,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(30772752);河北省自然科学基金资助项目(C2008000952)

摘　　要：目的:研究中药五味子(Schisandra Chinensis,SC)提取物对辐射所致小鼠免疫功能的影响,探讨SC对辐射所致免疫功能损伤的保护作用。方法:将48只BALB/c小鼠随机分为4组,分别为SC预处理组(SC灌胃后照射)、生理盐水组(NS灌胃后照射)、SC对照组(只给SC,不照射)和正常对照组(只给NS,不照射)。6 Gy^(60)Co照射后24 h进行外周血白细胞和淋巴细胞计数;流式细胞仪检测小鼠外周血中CD4^+、CD8^+T细胞的百分比,用免疫浊度法检测小鼠血清IgG及补体C3的含量。结果:与正常对照组相比,NS组照射24 h后外周血白细胞总数[(3.73±1.05)×10~9]和淋巴细胞数量[(2.06±0.57)×10~9]均明显下降(P<0.01),而SC预处理组白细胞总数[(6.43±0.76)×10~9]和淋巴细胞数量[(4.15±0.95)×10~9]较NS均明显升高(P<0.01);照射后小鼠外周血中CD3^+、CD4^+和CD8^+T细胞百分比、IgG及补体C3水平较正常对照组明显降低(P<0.01);SC预处理组较NS组小鼠外周血中CD4^+和CD8^+T细胞绝对值、IgG及补体C3水平均明显升高(P<0.01)。结论:SC提取物能够预防辐射所致淋巴细胞减少和免疫功能损伤。OBJECTIVE：To study the effects of Schisandra Chinensis（SC） extract on prevention and treatment of immune dysfunction caused by radiation.METHODS：48 BALB/c mice were divided into 4 groups,SC treated group（SC 4 mg/g ＋ 6 Gy for 396 s）,NS group（NS ＋ 6 Gy for 396 s）,SC control group（SC 4 mg/g ） and normal group（NS）.WBC and lymphocyte subset（LYM） of peripheral blood were counted.Percentages of CD4~＋ and CD8~＋ T cells were measured by flow cytometry.Contents of IgG and C3 in mouse serum were analyzed by immunological turbidimetry. RESULTS：After irradiation,the number of WBC and LYM in peripheral blood of NS-treated mice was lower than that of normal control group[（3.73±1.05）×10~9 and（2.06±0.57）×10~9,respectively,P 0.01].Compared to NS group,the number of WBC and LYM of SC treated mice was higher[（6.43±0.76）×10~9 and（4.15±0.95）×10~9,respectively, P 0.01].There was no significant difference between SC group and NS group（P 0.05）.The absolute numbers of CD4~＋ and CD8~＋ T subsets and the levels of IgG and C3 were decreased after radiation（P 0.01）.Compared to NS group,the number of T lymphocyte subsets in SC-treated mice was higher than that of NS group（P0.01）.CONCLUSION：SC could prevent the lymphocyte reduction and immune injury caused by radiation.

关 键 词：五味子 辐射 免疫功能 淋巴细胞 预防 

分 类 号：R818[医药卫生—放射医学]