氯氰菊酯对模式动物秀丽隐杆线虫生殖能力的损伤作用  

Reduction of reproductive capacity of model organism Caenorhabditis elegans induced by cypermethrin exposure

作　　者：阮秦莉[1] 居静娟[1] 李云晖[1] 李晓波[1] 刘冉[1] 梁戈玉[1] 张娟[1] 浦跃朴[1] 尹立红[1] 王大勇[2] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室,江苏南京210009 [2]东南大学医学院生物化学和分子生物学系,江苏南京210009

出　　处：《癌变．畸变．突变》2012年第2期136-140,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81072258;30901167);江苏省自然科学基金(BK2009281;BK2010402);国家教育部博士点专项基金(20090092120055)

摘　　要：目的:应用模式动物秀丽隐杆线虫研究菊酯类农药氯氰菊酯的生殖毒性,并对秀丽隐杆线虫模型在生殖毒理学中应用的可行性进行初步探讨。方法:将L4期野生型秀丽隐杆线虫分别于0.08、0.8和8.0 mg/L的氯氰菊酯中暴露24、48和72 h,每组10条,以K溶液为阴性对照,分别在体视显微镜和光镜下计数后代数目、子宫内受精卵数目、卵母细胞数目并观察形态,体外排受精卵速率。结果:L4期野生型秀丽隐杆线虫暴露于氯氰菊酯24 h后,各观察终点均无显著性变化;暴露48 h后,8.0 m/L浓度染毒组发生后代数目降低,0.8和8.0 m/L染毒组纳精囊旁侧第2位卵母细胞相对长度减小且子宫内受精卵数目降低,0.08、0.8和8.0mg/L染毒组体外排受精卵速率均下降,与对照组相比,差异均具有统计学意义(P<0.05);暴露72 h后,0.8和8.0 m/L染毒组后代数目降低,纳精囊旁侧第2位卵母细胞相对长度减小,与对照组相比,差异均具有统计学意义(P<0.05)。结论:模式动物秀丽隐杆线虫生殖系统对氯氰菊酯具有一定敏感性,在生殖毒理学研究中具有潜在应用价值。OBJECTIVE：To study the reproductive toxicity of cypermethrin exposure on Caenorhabditis elegans and explore the feasibility of using Caenorhabditis elegans as a model organism in reproductive toxicology. METHODS：L4 N2 hermaphrodites were exposed to cypermethrin at 0.08、0.8 and 8.0 mg/L for 24,48,and 72 h. RESULTS：After exposure of 24 h,there was no significant change in the endpoints observed.After exposure of 48 h,together with the decrease in brood size,decreased relative length of secondary-lateral seminal vesicle oocyte and the number of egg in utero,reduced rate of egg-laying were observed in nematodes exposed to cypermethrin at certain experimental concentrations.After exposure of 72 h,significant decreases in brood size and relative length of secondarylateral seminal vesicle oocyte were observed at 0.8 and 8.0 mg/L cypermethrin groups.CONCLUSION：Cypermethrin reduced the brood size through impairing the development of oocyte and egg.Meanwhile,Caenorhabditis elegans was sensitive to the exposure of cypermethrin and can be recommended to be used as a model organism in the field of reproductive toxicology.

关 键 词：氯氰菊酯 秀丽隐杆线虫 生殖毒性 生殖能力 

分 类 号：R994.6[医药卫生—毒理学]