转基因小麦T_1代中外源基因的检测和分析  

Detceting and analysis of foreign gene in T_1 generation of transgenic wheat

作　　者：周继阳[1] 祝长青[1] 覃建兵[1] 

机构地区：[1]新疆师范大学分子生物学与生物信息研究室,乌鲁木齐830053

出　　处：《华中师范大学学报（自然科学版）》2012年第2期204-207,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(30960092);(30960044);新疆师范大学研究生创新基金(20111211)

摘　　要：该研究采用Multiplex PCR和SDS-PAGE技术对转1 Dx5基因(ORF)T1代小麦进行了检测和分析.结果显示,Multiplex PCR能够扩增出转基因T1代材料中1 Dx2基因和1 Dx5基因的特征片段,表明外源基因已整合到受体基因组中;SDS-PAGE检测到一新的蛋白质亚基X,表明外源基因的插入引起了转基因T1代籽粒中HMW-GS组成的变化.该研究不仅验证了线性基因片段遗传转化策略的可行性,而且印证了普通小麦Glu-1D等位基因的多重PCR分子标记体系的有效性,为培育安全型的转基因作物新种质打下坚实的基础,加快小麦分子育种进程.In order to detect and analyze heritance of Foreign 1Dx5 （ORF） in T1 Generation of Transgenic Wheat （The ORF of 1Dx5 was used for transformation of wheat （Xindong-26） via pollen tube pathway）. First of all, Characteristic fragment of 1Dx2 and 1Dx5 in transgenic T1 lines by Multiplex-PCR show that foreign gene had been inserted into the genome of wheat cultivar;secondly, a novel protein X was tested in HMW-GS compositions by SDS-PAGE, that may be ascribed to insertion of foreign gene. To sum up, this GM strategy of linear gene is effective and feasible and the PCR- base marker system is available to distinguish between ordinary wheat GIu-ID alleles , which provides a solid foundation to speed up the process of molecular breeding of wheat.

关 键 词：转基因小麦 线性1Dx5核心片段 蛋白质检测 多重PCR 外源基因整合 

分 类 号：Q78[生物学—分子生物学]