莽草酸对血小板聚集和凝血的抑制作用  

INHIBITORY EFFECTS OF SHIKIMIC ACID ON PLATELET AGGRAGATION AND BLOOD COAGULATION

作　　者：马怡[1] 孙建宁[1] 徐秋萍[1] 郭亚建 

机构地区：[1]北京中医药大学药理教研室,北京100029

出　　处：《药学学报》2000年第1期1-3,共3页Acta Pharmaceutica Sinica

摘　　要：目的：研究莽草酸（ＳＡ）对血小板聚集性的影响及其机制，并研究其对凝血时间的影响。方法：采用比浊法测定血小板聚集功能，放射免疫法测定血小板ＴＸＢ２ 和血浆６ｋｅｔｏＰＧＦ１α水平，玻管法测定凝血时间。结果：ＳＡ 体外呈浓度依赖性抑制ＡＤＰ、胶原诱导的兔血小板聚集，ＩＣ５０ 分别为９－２５ ，３－５６ ｍ ｍｏｌ·Ｌ－１ ；在体内（ｉｐ） 抑制脑血栓模型鼠由ＡＤＰ诱导的血小板聚集；ＳＡ促进大鼠血浆６ｋｅｔｏＰＧＦ１α的生成并延长小鼠凝血时间。结论：ＳＡ 对血小板聚集及凝血系统有显著的抑制作用。AIM: To study the inhibitory effects of shikimic acid (SA) on platelet aggregation and its mechanism, and the effect on coagulation time. METHODS: Methods of turbidimetry and radioimmunoassay were used to measure platelet aggregation and the levels of thromboxane B 2(TXB 2) and 6 keto PGF 1α . RESULTS: SA inhibited rabbit platelet aggregation induced by ADP and collagen in vitro with IC 50 of 9 25 and 3 56 mmol·L -1 , respectively. SA 25 and 50 mg·kg -1 ip for 3 d inhibited platelet aggregation induced by ADP in rats subjected to middle cerebral artery thrombosis(MCAT). SA was shown to increase rat plasma 6 keto PGF 1α and no significant effect on TXB 2 generation in PRP induced by ADP. SA 25, 50 and 100 mg·kg -1 im or iv elevated the blood coagulation time of mice. CONCLUSION: SA inhibited platelet aggregation and blood coagulation.

关 键 词：莽草酸 血小板聚集 血栓素B2 血液凝固 中药 

分 类 号：R286.32[医药卫生—中药学] R285.5[医药卫生—中医学]