北沙参的碳-13核磁共振指纹图谱研究  

Identification of Glehnia littoralis by ^(13)CNMR fingerprint

作　　者：刘静[1] Dan Staerk 孙庆雷[1] 李恩霞[1] 赵梅[1] 马广存[1] 江婷[1] 吕瑞敏[1] 

机构地区：[1]山东省分析测试中心,山东济南250014 [2]哥本哈根大学生命科学学院基础科学环境系

出　　处：《山东科学》2012年第2期26-29,共4页Shandong Science

基　　金：山东省海外高层次人才资助专项资金

摘　　要：利用统一的方法获得了反映北沙参整体特征的特征提取物。应用超导脉冲傅里叶变换核磁共振波谱仪测定了不同产地北沙参特征提取物的碳-13核磁共振指纹图谱,并采用向量夹角余弦法计算了该指纹图谱的相似度。研究结果表明,不同北沙参提取物的碳-13核磁共振指纹图谱显示北沙参包含多种成分的共振峰,其特征有效成分的特征共振峰有很高的重现性和较高的特征性。通过北沙参提取物的碳-13核磁共振指纹图谱及其特征区域的共振峰的有效结合,可以做为北沙参真伪鉴别的依据。We employed a universial extract approach to obtain Glehnia fittoralis extracts reflecting the their total characteristics. We further applied a Fourier transform nuclear magnetic resonance spectrometer to determine the Carbon-13 Nuclear Magnetic Resonance （13CNMR） fingerprints of different Glehnia littoralis extracts and calculated their similarities with vectorial angle method. Results show that the resonant peaks of many components exist in the fingerprints of different Glehnia littoralis extracts. The characteristic resonant peaks of their active components exhibit better repeatability and characteristics. A joint analysis of 13CNMR fingerprints and their characteristic peaks would provide an identification basis for Glehnia littoratis.

关 键 词：碳-13核磁共振 指纹图谱 北沙参 鉴别 

分 类 号：O697.61[理学—化学] R282.5[医药卫生—中药学]