转Fat-1基因体细胞克隆草原红牛研究  

Production of Fat-1 transgenic chinese red steppe by somatic cell cloning

作　　者：康锦丹[1] 高威威[1] 高青山[1] 张立春[2] 

机构地区：[1]延边大学动物胚胎工程研究所,吉林延吉133002 [2]吉林省农业科学院,吉林长春130030

出　　处：《延边大学农学学报》2012年第1期6-10,20,共6页Agricultural Science Journal of Yanbian University

基　　金：转基因生物新品种培育重大专项(2009ZX08007-005B);吉林省自然科学基金项目(20101590)

摘　　要：Fat-1基因的转基因动物克隆,为研究ω-3多不饱和脂肪酸的功能提供了高效、准确的医学、营养学动物模型。试验成功建立了草原红牛耳部成纤维细胞系,将Fat-1基因转染到草原红牛成纤维细胞,获得转基因阳性细胞克隆。以转基因细胞为核供体,体外成熟牛卵母细胞为核受体构建转基因克隆囊胚,比较未转基因与转基因克隆胚胎的体外发育情况。结果表明,体细胞重构胚体外囊胚率分别为26.04%和26.04%,两者之间无显差异著(P>0.05)。转Fat-1基因的草原红牛重构桑葚胚或早期胚胎移植到延边黄牛子宫内,有7头妊娠,但没能够获得妊娠足月个体。Production of Fat-1 transgenic animal supplied the effective animal model for the study of the function of ω-3 polyunsaturated fatty acid.The study established the ear fibroblasts of Steppe Red Cattle and transfected the Fat-1 gene into them,then get the cloned transgenic cell.Make the transgenic cell as donor cell and the in vitro maturaed oocyte as nuclear receptor to reconstruct the transgenic blastula,compared the development of the non-transgenic and transgenic embryo.The results showed that the rate of reconstructed embryo was 26.04% and 26.04%,there was no significant difference（P〉0.05）.Transferred the Fat-1 transgenic reconstructed morula or early embryo into the Uterus of Yanbian yellow cow,7 of them were pregnant but didn＇t develop to full term.

关 键 词：Fat-1基因 体细胞克隆 草原红牛 

分 类 号：S823[农业科学—畜牧学]