延边膜荚黄芪rDNA ITS区域克隆与序列分析  

Cloning and sequence analysis of rDNA ITS region of Yanbian Astragalus membranaceus

作　　者：金钟范[1] 王树斌[1] 杨洪亮[2] 邱菊[2] 吴松权[1] 

机构地区：[1]延边大学农学院,吉林延吉133002 [2]吉林省大安市农业技术推广中心,吉林大安131300

出　　处：《延边大学农学学报》2012年第1期27-29,共3页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金项目(30860036);吉林省科技厅项目(201115228);延边大学科技项目(延大科合字[2010]第14号)

摘　　要：用PCR方法克隆延边地区膜荚黄芪的核糖体DNA ITS区域并进行序列分析。结果表明:延边地区膜荚黄芪ITS1的序列长度为228bp,5.8SrDNA长度为164bp,ITS2的序列长度为210bp;序列分析表明延边地区和甘肃的ITS1和5.8SrDNA完全一致,而ITS2第82位处有1个碱基的置换(T/C)。With the method of PCR and bioinformatics,rDNA ITS region of Astragalus membranaceus from Yanbian was cloned and analyzed.The results showed that the length of rDNA ITS1,5.8 S rDNA and rDNA ITS2 for A.membranaceus from Yanbian were 228 bp,164 bp and 210 bp respectively,no difference was observed in rDNA ITS1 and 5.8 S rDNA sequences,but one base inversion（T / C）was observed at the 82nd sequence in rDNA ITS2 between Yanbian and Gansu.

关 键 词：膜荚黄芪 RDNA 内转录间隔区(ITS) 

分 类 号：S567[农业科学—中草药栽培]