超声提取—气相色谱法测定油脂中胆固醇  

DETERMINATION OF CHOLESTEROL IN OIL BY ULTRASONIC EXTRACTION-GAS CHROMATOGRAPHIC

作　　者：潘红红[1] 姜涛[2] 周易 陈代伟 

机构地区：[1]成都理工大学材料与化学化工学院,四川成都610059 [2]国家粮食局成都粮油食品饲料质量监督检验测试中心,四川成都610091 [3]成都市产品质量监督检验院,成都610041

出　　处：《粮食储藏》2012年第2期34-37,共4页Grain Storage

摘　　要：建立了毛细管气相色谱法测定油脂中胆固醇含量的方法,改进了油脂皂化方法以及皂化完成后使用分液漏斗提取胆固醇过程中容易产生乳化、操作复杂且比较耗时的缺点,采用超声波提取胆固醇,优化了样品前处理条件。结果表明,该法在0.002 mg/mL～0.500 mg/mL范围呈线性(r=0.9997),检出限为20 mg/kg,RSD=1.104%,回收率在81.3%～93.8%之间。A method was established for the determination of cholesterol in oil by capillary gas chromatography,which improved oil saponification method and overcame the shortcomings that separatory funnel extraction of cholesterol was prone to emulsification and the operation was complex and time-consuming after saponification.Also,the use of ultrasonic extraction of cholesterol optimized the sample of pre-treatment conditions.The results showed that the method was suitable for detection,RSD= 1.104%,When cholesterol concentration was in fine linear relation（r=0.9997） with chromatogram peak area with in the range of 0.002～0.5 mg/mL,the detection limit of method was 20 mg/kg,the average recovery was 81.3%-93.85%.The method of detection of cholesterol in oil was economical,simple,rapid and efficient.

关 键 词：油脂 气相色谱法 超声提取 胆固醇 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.71[轻工技术与工程—食品科学与工程]