湿加松亲本间遗传距离与杂种优势的相关性分析  

Relationship between Growth Traits Heterosis and Genetic Distance among Parents of Pinus elliottii × P.caribaea Based on SSR Molecular Markers

作　　者：李义良[1] 赵奋成[1] 吴惠姗[1] 张应中[1] 李福明 钟岁英 李宪政[1] 蔡坚[1] 

机构地区：[1]广东省林业科学研究院林业研究所,广东广州510520 [2]广东省台山红岭种子园,广东台山529200

出　　处：《林业科学研究》2012年第2期138-143,共6页Forest Research

基　　金：国家林业局林业公益性行业科研专项(项目编号:201104014);广东省林业科技创新专项资金项目(项目编号:2008KJCX005-01;2009KJCX007-01;2010KJCX007-01);广东省科技厅(2007A020200001-1);北京林业大学林木育种国家工程实验室开放课题项目(FOP2010-3)共同资助

摘　　要：利用SSR标记对17个湿加松亲本无性系进行遗传多样性分析,进而分析亲本遗传距离与子代杂种优势的相关性。结果表明:14个微卫星标记在湿加松亲本群体中均呈现高度多态,共检测到57个等位基因,多态位点百分率为87.72%,平均杂合度为0.144 4 0.884 1,以SSR标记遗传距离聚类,将参试亲本材料划分为2大类群,可以用于湿加松亲本遗传多样性的评价。树高、胸径、材积的杂种优势与亲本遗传距离相关性达显著或极显著水平,相关系数分别为0.542 0、0.641 2、0.639 3,决定系数分别为0.293 8、0.411 0、0.408 7。分子标记遗传距离与树高、胸径、材积的杂种优势相关程度较高,利用SSR标记预测湿加松杂种优势是可行的,为湿加松良种选育及亲本选配提供参考。Seventeen Pinus elliottii × P.caribaea parental lines were assayed by Simple Sequence Repeat（SSR） to evaluate the genetic diversity and determine the correlation between genetic distance and heterosis.The results indicated that there were abundant genetic polymorphisms at 14 SSR molecular markers in parents of hybrid pine,57 alleles were detected in total,the percentage of polymorphic loci was 87.72%,and the average heterozygosity ranged between 0.144 4 and 0.884 1,the microsatellite loci could be used for genetic diversity evaluation in parents of hybrid pine.The results showed that there was significant relation or extremely significant relation between genetic distance and height,DBH and volume of heterosis.The correlative coefficients were 0.542 0,0.641 2 and 0.639 3,and the determination coefficient were 0.293 8,0.411 0 and 0.408 7.Since there existed a relative closer correlation between the genetic distance and height,DBH and volume of heterosis,it is feasible to predict the hybrid pines heterosis by SSR markers.

关 键 词：湿加松 SSR标记 遗传距离 杂种优势 

分 类 号：S718.46[农业科学—林学]