平榛ChWRKY2转录因子的克隆及在低温胁迫下的表达分析  

Cloning and Expression Analysis of ChWRKY2 from Corylus heterophylla under Low Temperature Stress

作　　者：赵天田[1] 王贵禧[1] 梁丽松[1] 马庆华[1] 陈新[1] 

机构地区：[1]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,北京100091

出　　处：《林业科学研究》2012年第2期144-149,共6页Forest Research

基　　金：国家"十一五"科技支撑计划"高产优质大枣;榛子新品种选育"(2006BAD01A1701);国家林业局"948"项目"榛子特异性状育种材料及微繁技术引进"(2008204208)

摘　　要：根据平榛花芽转录本高通量测序的结果,采用RACE-PCR技术克隆到一个全长675 bp,编码179个氨基酸的平榛WRKY基因,命名为ChWRKY2。ChWRKY2蛋白序列中只含有一个WRKY结构域,锌指结构为C-X4-C-X23-H-X1-H,属于WRKY家族第Ⅱ类成员。对ChWRKY2基因进行实时荧光定量PCR表达分析,结果表明:平榛雌花芽中ChWRKY2在12月份的表达量最高,随后表达量逐渐下降。4℃低温胁迫处理根蘖苗4 h后,叶片中ChWRKY2的表达量快速升高,并在8 h达到最大表达量。此外,ChWRKY2在不同器官中的表达具有差异性,雄花序中表达量最高,其次是雌花芽,树皮(韧皮部)中表达量最低。According to the high-throughput sequencing results of hazelnut buds,a 675 bp fragment,ChWRKY2,which encoded a polypeptide of 179 amino acids was obtained through RACE-PCR.The deduced protein sequence shows that this protein belongs to the second group of WRKY family and the zinc-finger structure is C-X4-C-X23-H-X1-H.The ChWRKY2 expression in hazelnut buds was analyzed under natural conditions and the result displayed that the expression level was the highest in December and then decreased.ChWRKY2 in leaves were rapidly and highly induced when suckers were exposed to low temperatures of 4 ℃,ChWRKY2 were activated at 8 h reaching a peak.the spatial expression analysis revealed that the transcription level of ChWRKY2 was relatively higher in male inflorescence than that in bud and bark tissues.

关 键 词：平榛 WRKY基因 克隆 序列分析 实时荧光定量PCR 

分 类 号：S718.46[农业科学—林学]