欧美杨渤丰1号高效组培再生体系建立  

Establishment of an Efficient Regeneration System of Populus × euramericana cl.'Bofeng 1'

作　　者：崔旭东[1] 苏晓华[1] 张冰玉[1] 褚延广[1] 黄秦军[1] 张伟溪[1] 刘勃洋[2] 

机构地区：[1]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,北京100091 [2]北京林业大学生物科学与技术学院,林木花卉遗传育种教育部重点实验室,北京100083

出　　处：《林业科学研究》2012年第2期157-162,共6页Forest Research

基　　金：林业公益性行业科研专项(201004004);"863"国家高技术研究发展计划项目(2011AA100201)

摘　　要：与白杨派树种相比,黑杨派树种一直因组培再生困难而限制了其在林木基因工程研究中的应用。本文以欧美杨优良新品种———渤丰1号为试验材料,以其再生培养基中的植物生长调节剂配比、铜离子浓度、光照强度、卡那霉素选择压等方面为切入点开展研究,建立了渤丰1号杨高效组培再生体系。使用该再生体系时,外植体的分化率和生根率均达100%,叶片的平均分化芽数多达20个以上,组培苗移植成活率可达98%,40 mg.L-1卡那霉素可以抑制渤丰1号杨叶片的诱导与分化,20 mg.L-1卡那霉素可以抑制渤丰1号杨不定芽的生根;同时发现铜(Cu)元素是渤丰1号杨外植体分化再生的一个关键因素,增加Cu2+浓度能显著促进不定芽的诱导和分化。Compared with the species of Leuce section,the difficulty in plant regeneration during tissue culture for the species of Aigeiros section is a key limiting factor for their application in genetic engineering of forest trees.In this study,the plant growth regulators combination,copper concentration,light intensity,and the selection pressures of kanamycin were investigated using leaves of Populus × euramericana cl.＇Bofeng 1＇ as explants,and a stable and high efficient regeneration system was established.Using this system,both the shoot regeneration rate and rooting rate were up to 100%,and the average number of differentiated shoots in each leaf explant was up to 20,and the survival rate of seedlings reached 98%.The optimal selection pressure of kanamycin in shoot inducement for leaf-explant was 40 mg·L-1,and the optimal selection pressure of kanamycin in root inducement of adventitious bud was 20 mg·L-1.The authors also found that differentiation rate of adventitious buds of Populus × euramericana cl.＇Bofeng 1＇ could be significantly promoted by increased copper concentration,and this is the first report about the key role of copper（Cu） in explant regeneration of Poplars.This regeneration system with high frequency which was established by the authors was as good as that of species of Leuce section,and it provides a good technological platform for genetic engineering of forest tree which uses the species of Aigeiros section as model materials.

关 键 词：欧美杨渤丰1号 高效再生体系 铜元素 

分 类 号：S722.3[农业科学—林木遗传育种]