杜仲1-脱氧-D-木酮糖-5-磷酸还原异构酶基因cDNA全长克隆与序列分析  

Cloning and Sequence Analysis of 1-Deoxy-D-Xylulose 5-Phosphate Gene cDNA from Eucommia ulmoides

作　　者：刘攀峰[1] 杜红岩[1] 乌云塔娜[2] 黄海燕[1] 朱高浦[1] 

机构地区：[1]中国林业科学研究院经济林研究开发中心,河南郑州450003 [2]国家林业局经济林育种与栽培重点实验室,湖南长沙410004

出　　处：《林业科学研究》2012年第2期195-200,共6页Forest Research

基　　金：国家公益性行业科研专项(201004029)

摘　　要：以杜仲叶片cDNA为模板,采用反转录RCR及RACE技术分离出DXR基因cDNA全长。序列分析结果表明该基因序列全长1 814 bp,共编码478个氨基酸,推导的蛋白质分子量为51.71 kD,理论等电点5.79,命名为EuD-XR。推导的EuDXR蛋白质序列具有植物DXR酶的5个典型基序,并预测出26个潜在的功能位点。系统进化树分析表明EuDXR蛋白与玉米、水稻亲缘关系最近,其次为橡胶、拟南芥、烟草。Homologous DXR gene cDNA was isolated from leaves of Eucommia ulmoides by method of reverse transcription polymerase chain reaction（RT-PCR） and rapid amplification of cDNA ends（RACE） technique,and named after EuDXR.The nucleotide sequence of EuDXR was 1 814 bp and contains an open reading frame（ORF） of 1 437 bp encoding 478 amino acids with a molecular weight of about 51.71 kD,and the theoretical isoelectric point of which was 5.79,representative motifs of plant DXR and 26 potential functional sites were deduced in the amino acids sequence of EuDXR.The results of phylogenetic analysis suggested that the protein sequence of EuDXR was more similar to that of Zea mays and Oryza sativa than other species,and followed by Hevea brasiliensis,Arabidopsis thaliana and Nicotiana tabacum.

关 键 词：杜仲 DXR 基因 序列分析 

分 类 号：S718.46[农业科学—林学]