中国南极越冬队员外周血生物钟基因Clock和Bmal1昼夜性节律表达赴南极前后对比  

Comparison of Circadian Expression of Biological Clock Gene,Clock and Bmal 1,in Peripheral Blood Lymphocytes of Chinese Antarctic Wintering Team Members before and after Antarctic Expedition

作　　者：余万霰[1] 陈绍平[2] 夏艳芝[3] 王国卿[3] 王洁[3] 张永虹[4] 

机构地区：[1]南昌大学医学实验教学部,南昌330006 [2]南昌大学第四附属医院骨科,南昌330006 [3]苏州大学医学部基础医学院生理教研室,江苏苏州215123 [4]南昌大学第二附属医院检验科,南昌330006

出　　处：《南昌大学学报（医学版）》2012年第2期1-5,共5页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(30760069);中国第25次南极考察现场执行计划(中山站)

摘　　要：目的观察中国南极考察队越冬队员外周血淋巴细胞钟基因Clock和Bmal 1表达昼夜节律性变化。方法在中国第25次南极考察队越冬队员中选择8名队员,平均年龄38岁,均为男性,在1个昼夜周期内设立6个时点(ZT):02:00、06:00、10:00、14:00、18:00和22:00,每一时点采集外周静脉血6mL,采集赴南极前后2组血样。用实时定量RT-PCR方法,测定不同昼夜时点(ZT)样品中核心钟基因Clock和Bmal 1的mRNA表达量,通过余弦法和Clock Lab软件获取节律参数,进行赴南极前后对比。结果赴南极前,8个样本Clock和Bmal 1的mRNA表达均有显著昼夜节律特征(P<0.05),Clock的峰值相位位于-335.85±13.80,Bmal 1的峰值相位位于-307.12±8.17。赴南极后,仅2例Clock和3例Bmal 1表达还存在显著昼夜节律变化。Clock的峰值相位移位到-42.28±5.27,Bmal 1的同峰值相位移位到-184.58±29.58。结论南极特殊周期环境对人体生物钟基因表达的昼夜节律会产生影响。Objective To investigate the circadian expression of core clock gene,Clock and Bmal 1,in the peripheral blood lymphocytes of Chinese scientific expeditioners during the Antarctic winter.Methods Peripheral blood（6 mL） were collected in 8 healthy male volunteers（mean age 38 years） from wintering team of the 25th Chinese Antarctic Research Expedition at 6 time points in the day-night cycle（02：00,06：00,10：00,14：00,18：00 and 22：00）.In addition,blood samples were collected before and after Antarctic expedition.The expression of Clock and Bmal 1 at different Zeitgeber times（ZT） was detected by RT-PCR.The circadian parameters were obtained and analyzed by the cosine function,Clock Lab software and amplitude F test for comparison before and after Antarctic expedition.Results Before Antarctic expedition,the expression of Clock and Bmal 1 mRNA showed a significant circadian feature（P0.05）.The Peak phase of Clock at-335.85±13.80.The Peak phase of Bmal 1 at-307.12±8.17.After Antarctica,the mRNA transcriptions of 2 expeditioners＇ Clock and 3 expeditioners Bmal 1 continuously sustain the significant circadian oscillation among the same 8 Chinese scientific expeditioners.The Peak phase of Clock is at-42.28±5.27,The Peak phase of Bmal 1 is at-184.58±29.58.Conclusion Antarctic environment exerts an impact on human circadian clock gene expression.

关 键 词：CLOCK基因 Bmal1基因 昼夜节律 外周血淋巴细胞 南极 越冬队员 中国 

分 类 号：R349.64[医药卫生—基础医学]