利用RNAi和表达谱芯片分析日本血吸虫Sj34.9基因的功能  

Function analysis of Sj34.9 gene based on RNAi and microarray

作　　者：刘萍萍[1] 石艳丽[1] 杨云霞[1] 刘金明[1] 林矫矫[1] 金亚美[1] 

机构地区：[1]中国农业科学院上海兽医研究所、国家防治动物血吸虫病专业实验室、农业部动物寄生虫学重点开放实验室,上海200241

出　　处：《中国血吸虫病防治杂志》2012年第2期127-131,145,共6页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(30800819);上海市浦江人才计划(09PJ14118);中央级公益性科研院所基本科研业务费(2010JB11)

摘　　要：目的分析日本血吸虫新基因Sj34.9的功能,为进一步研究该基因提供依据。方法采用RNA干扰(RNAi)技术对Sj34.9基因在血吸虫体内的表达进行干扰,利用血吸虫寡聚核苷酸表达谱芯片分析Sj34.9基因表达沉默后血吸虫基因的表达情况。结果 Sj34.9基因RNAi组与对照组相比,显著差异表达的基因共378个,其中上调基因202个,下调基因176个;通路分析显示,表达差异基因主要与信号转导、信号分子间相互作用及各类物质代谢有关;基因本体论分类结果显示,多数基因与分子间结合、膜构成、细胞过程和机体生长发育及代谢相关。结论 Sj34.9基因可能对日本血吸虫的形成、生长、发育、调节、代谢等具有重要作用。Objective To analyze the function of Sj34.9 gene,so as to provide the reference for future studies.Methods The Sj34.9 gene was knocked down in Schistosoma japonicum by RNA interference（RNAi）,and the microarray was used to analyze the genes＇expression of S.japonicum after Sj34.9 knocked down.Results A total of 378 genes expressed differently including 202 up-regulated genes and 176 down-regulated genes.The pathway analysis indicated that the genes expressed differently were mainly related to organelles,metabolism and signal transduction.The gene ontology category analysis showed that most of these genes might be involved in binding,membrane fomulation and cellular process.Conclusion The gene Sj34.9 might play important roles in the process of growth,development,reproduction and metabolism of S.japonicum.

关 键 词：日本血吸虫 RNA干扰(RNAI) 表达谱芯片 基因表达 

分 类 号：R383.24[医药卫生—医学寄生虫学]