hVEGF165及hBMP-7共表达重组腺相关病毒载体对兔激素性股骨头坏死的修复作用研究  

The effects of recombinant adeno-associated virus vector co-expressing hVEGF165 and hBMP-7 genes in steroids-induced avascular necrosis of femoral head

作　　者：张晨[1] 王坤正[1] 党晓谦[1] 王春生[1] 杨佩[1] 樊立宏[1] 

机构地区：[1]西安交通大学医学院第二附属医院骨一科,710004

出　　处：《中华关节外科杂志（电子版）》2012年第2期51-54,共4页Chinese Journal of Joint Surgery(Electronic Edition)

基　　金：国家自然科学基金资助项目(30600624;81101363)

摘　　要：目的探讨hVEGF165及hBMP-7双基因共表达重组腺相关病毒载体对兔激素性股骨头坏死的修复作用。方法应用细菌脂多糖联合甲基强地松龙制备兔激素性股骨头坏死模型。30只经MRI筛选造模成功动物随机分为模型组、髓芯减压组和病毒治疗组。病毒治疗组动物于髓芯减压术后将rAAV-hVEGF165-IRES-hBMP-7病毒载体注入减压区内。选取病毒注射后12周时间点分别行核素骨扫描,股骨头大体观察、HE染色、VEGF和BMP免疫组化染色检测。结果 MRI检测、大体观察以及HE染色发现早期股骨头坏死动物模型建立成功,且病毒治疗组较髓芯减压组及模型组相比股骨头坏死表现明显减轻,VEGF及BMP表达增强,髓腔组织代谢旺盛,组间差别有统计学意义(P<0.05)。结论重组腺相关病毒载体rAAV-hVEGF165-IRES-hBMP-7通过增加髓腔组织的血运、增强股骨头区骨组织质量提高激素性股骨头坏死区骨修复能力。Objective To investigate the effects of recombinant adeno-associated virus vector co-expressing hVEGF165 and hBMP-7 genes in steroids-induced avascular necrosis of femoral head.Methods The rabbit model of femoral head necrosis was constructed by combination use of lipopolysaccharide and methylprednisolone.30 adult rabbits with femoral head necrosis selected by MRI were randomly divided into three groups as follows：model group,core decompression group and virus treatment group.rAAV-hVEGF165-IRES-hBMP-7 virus vector was injected into core decompression region after core decompression operation in the treatment group.The femoral head was examined by radionuclide scan,gross observation,histopathological staining,and immunohistochemistry staining for VEGF and BMP at 12 weeks after virus injection.Results Early animal model of femoral head necrosis was successfully established according to MRI,gross observation and HE staining.Comparing with model group and core decompression group,in virus treatment group,necrosis of femoral head was significantly reduced;VEGF and BMP expressions were increased;and the metabolism of bone marrow tissue was exuberant.The differences of these three groups were statistically significant（P0.05）.ConclusionsThe recombinant adeno-associated virus vector rAAV-hVEGF165-IRES-hBMP7 can improve bone repair capacity in necrosis region of femoral head by increasing the blood supply of cavum medullare tissue and improving the bone quality of femoral head.

关 键 词：股骨头坏死 腺相关病毒 血管内皮生长因子 骨形态发生蛋白 兔 

分 类 号：R681.8[医药卫生—骨科学]