超声提取-高效液相色谱法测定麻黄中的麻黄碱  

Determination of ephedrines in Ephedra sinica by HPLC with ultrasonic extraction

作　　者：李长缨[1] 李东方[2] 李庆华[3] 黄九林[4] 魏春雁[5] 

机构地区：[1]重庆大学医院,重庆400045 [2]沈阳市疾病预防控制中心,沈阳110031 [3]吉林医药学院,吉林132013 [4]安康学院,陕西安康725000 [5]吉林省农业科学院,长春130033

出　　处：《中国卫生检验杂志》2012年第4期707-708,710,共3页Chinese Journal of Health Laboratory Technology

基　　金：吉林省科技发展计划重点资助项目(20040901-02-01)

摘　　要：目的:优选麻黄中麻黄碱、伪麻黄碱和甲基麻黄碱的最佳提取工艺和高效液相色谱定量方法。方法:用超声波辅助提取法优选最佳提取工艺;用高效液相色谱法对麻黄中麻黄碱、伪麻黄碱和甲基麻黄碱进行含量测定。结果:最佳提取工艺为超声波辅助水提取法,超声提取时间30 min;HPLC最佳定量测定条件为:C18柱(4.6 mm×250 mm;5μm)、流动相0.02 mol/L NaH2PO4:甲醇:三乙胺(99.8:0.2:0.2)、流速1.5 ml/min、进样量为20μl、温度为室温、检测波长为210 nm。结论:该提取工艺合理,含量测定方法简单、可靠。Objective：To optimize the conditions for extraction and measurement of ephedrine,methylephedrine and pseudoephedrine in Ephedra sinica by HPLC with ultrasonic extraction.Methods：Ultrasonic technique was applied to extract ephedra alkloids and HPLC method was used to determine the contents of ephedrine,methylephedrine and pseudoephedrine in E.sinica.Results：Ultrasonic extraction with water was suitable for the 3 target components from E.sinica.And the best HPLC determination condition for ephedrine,methylephedrine and pseudoephedrine in E.sinica separation was：column C18,mobile phase 0.02 mol/L NaH2PO4：methanol：triethylamine（99.8：0.2：0.2）,flow rate 1.5 ml/min,detection wavelength 210 nm.Conclusion：The method is suitable for the determination of ephedrines in Ephedra sinica.

关 键 词：麻黄 麻黄碱 伪麻黄碱 甲基麻黄碱 超声波辅助提取 高效液相色谱 

分 类 号：O657.72[理学—分析化学]