大豆苯丙氨酸代谢途径关键酶基因的挖掘定位及结构分析  

Gene Mining and Structure Analysis of Key Enzyme Genes on Phenylalanine Acid Metabolic Pathway in Soybean

作　　者：杜翔宇[1] 刘春燕[2] 吴琼[1] 蒋洪蔚[2] 辛大伟[1] 陈庆山[1,3] 栾怀海[2] 胡国华[2,3] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090 [3]国家大豆工程技术研究中心,黑龙江哈尔滨150050

出　　处：《大豆科学》2012年第2期178-183,共6页Soybean Science

基　　金：国家自然科学基金项目(30871551);现代农业产业体系(CARS-04-02A);国家转基因专项(2011ZX08004-001)

摘　　要：利用大豆基因组物理图和遗传图的整合图谱,应用blast软件将基因序列与大豆基因组数据库进行比对,完成了9个大豆天冬氨酸代谢途径中关键酶基因的定位以及结构分析。结果表明:9个基因分别定位在大豆的13个连锁群D1a、B1、N、I、O、C1、C2、F、L、A2、J、D1b以及B2上,并获得了基因所在序列两侧标记。利用大豆的cDNA和gDNA序列信息,获得了9个基因的结构,外显子数目为5～12个,内含子数目为4～11个。Synthesis of key enzyme genes on protein metabolic in soybean is quantitative traits controlled by multiple genes.Genetic markers and structural information with associated genes of plant protein synthesis can be obtained through mining the information of the key enzyme on protein synthesis in Arabidopsis.In this research,the soybean public genetic maps and physical maps ware integrated,and the key enzyme gene sequences were blasted with soybean genome database by local blast software.Nine key enzyme genes in phenylalanine acid metabolic pathway of soybean were mapped on soybean genetic map.The result indicated that these 9 enzyme genes were mapped on 13 linkage groups,including D1a,B1,N,I,O,C1,C2,F,L,A2,J,D1b,and B2,respectively.And the flanking markers of these genes on the linkage group were obtained.Furthermore,the sequence information between cDNA and gDNA was compared,the number of exon was from 5 to 12,and the number of intron was from 4 to 11.The corresponding markers obtained from the mapping are available for molecular assisted selection,and the structure information can be used in further gene function analysis.

关 键 词：大豆 苯丙氨酸 基因 定位 基因结构 

分 类 号：S565.1[农业科学—作物学]