农杆菌介导不同基因型大豆品种子叶节遗传转化条件的研究  

Optimization on Genetic Transformation Conditions of Agrobacterium-mediated Cotyledonary-nodes from Different Genotype Soybeans

作　　者：杨喆[1,2] 唐晓飞[1] 张颖[1] 董兴月[1] 王凤义[2] 刘丽君[1] 

机构地区：[1]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2012年第2期188-192,共5页Soybean Science

基　　金：转基因生物新品种培育重大专项资助项目(2008ZX08004003-005)

摘　　要：以黑农46、黑农53和黑农56的子叶节为转化受体,对农杆菌介导大豆子叶节遗传转化中的6-BA浓度、草丁膦浓度、侵染时间、共培养时间以及伸长培养基进行筛选,确定适合不同基因型的最佳转化条件。结果表明:黑农46、黑农53和黑农56的最佳6-BA诱导浓度分别为1.7、1.6和1.7 mg.L-1;最佳草丁膦筛选浓度分别为2.0、3.0和2.0 mg.L-1;同时确定了侵染时间、共培养时间和伸长培养基类型的最佳组合是侵染时间25 min、共培养时间3 d和Ⅲ型伸长培养基。Cotyledonary-nodes of Heinong 46,Heinong 53 and Heinong 56 were used as transformation receptors.Culture conditions including 6-BA concentration,glufosinate concentration,infection time,co-culture time and type of elongation medium were screened,in order to find the optimal conditions for Agrobacterium-mediated genetic transformation from different soybean genotypes.Result showed the optimal 6-BA induction concentrations for Heinong 46,Heinong 53 and Heinong 56 were 1.7,1.6 and 1.7 mg·L-1,respectively;the optimal concentrations of glufosinate for Heinong 46,Heinong 53 and Heinong 56 were 2.0,3.0,and 2.0 mg·L-1,respectively;the optimal combination of infection time,co-culture time and type of elongation medium was infection time 25 min,co-culture time 3 d and type Ⅲ of elongation medium.

关 键 词：大豆 不同基因型 农杆菌介导 遗传转化条件 

分 类 号：S565.1[农业科学—作物学]