混合表面活性剂增敏的石墨炉原子吸收法测定血清铬的研究  

Determination of chromium in serum with graphite furnace atomic absorption spectrometry

作　　者：夏淑琦[1] 吴晓鸥[1] 潘利琴[1] 张德亭[1] 陈维增[1] 罗涛[1] 

机构地区：[1]浙江省温州市第三人民医院检验科,浙江温州325035

出　　处：《中国卫生检验杂志》2012年第4期727-729,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立混合表面活性剂增敏的石墨炉原子吸收法测定血清铬的新方法,并应用于健康人和孕妇血清铬的测定。方法:血清用1.0%TritonX-100和0.6%Tween80组成的混合表面活性剂稀释后,以硝酸钯为基底改进剂,石墨炉原子吸收法测定血清中铬含量。结果:该法的线性范围为0.12μg/L～6.00μg/L,平均回收率为99.9%,批内和批间的相对标准偏差(RSD)分别为2.64%～4.81%和3.11%～5.32%。结论:用混合表面活性剂增敏的石墨炉原子吸收法测定血清铬,方法灵敏度高、重复性及准确性好,适合临床推广应用。Objective：To establish a new method for detecting chromium in serum.Methods：The serum was diluted by mixed surfactant composed of 1.0% TritonX-100 and 0.6% Tween80.With Pd（No3）2 as matrix modifiers,serum chromium content was determined by graphite furnace atomic absorption spectrometry.Results：The method was linear in 0.12 μg/L^6.00 μg/L,the average rate of recovery was 99.9%,the withinrun and betweenrun RSD were 2.64%~4.81% and 3.11%~5.32%.Conclusion：This method was suitable for clinical application with high sensitivity,good repeatability and accuracy.

关 键 词：血清 表面活性剂 石墨炉原子吸收 铬 

分 类 号：O657.31[理学—分析化学]