石墨炉原子吸收法测定全血铅的研究  

Measurement of Pb in whole human blood by Graphite Furnace Atomic Absorption Spectrometry

作　　者：卢洁[1] 李君[1] 许锴[1] 

机构地区：[1]浙江省温州市第三人民医院,浙江温州325000

出　　处：《中国卫生检验杂志》2012年第4期735-736,738,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立石墨炉原子吸收检测全血铅的方法。方法:以硝酸钯为基体改进剂,5%硝酸进行酸化,离心脱蛋白,石墨炉原子吸收法测定全血中铅含量。结果:该法的线性范围为0.00μg/L～80μg/L,检测限1.0μg/L,加标回收率为97%～104%,相对标准偏差(RSD)为3.0%～7.2%。结论:该方法简便、灵敏度高、重复性及准确性好,适合临床推广应用。Objective：To establish an atomic absorption spectrometry for content determination of Pb in whole human blood.Methods：Using palladium nitrate as matrix modifiers,samples were acidified with 5% nitric acid,centrifuged for deproteinization,then determined by atomic absorption method.Results：The linear range of calibration curve for the method was 0 μg/L^80 μg/L with correlation coefficient 0.9992,determination limit was 1.0 μg/L,recovery was 97%~104%,ralative standard deviation（RSD） was 3.0%~7.2%.Conclusion：The method was convenient,showing high sensetivity,repeatability and accuracy,which was suitable for clinical promotion.

关 键 词：全血铅 石墨炉原子吸收 基体改进剂 

分 类 号：O657.31[理学—分析化学]