克雷伯菌在不同培养基上生长的形态学特征分析研究  

Study on morphology characteristic of Klebsiella colony on the different culture medium

作　　者：魏琳琳[1] 翟育忠[1] 胡萍[1] 王新华[1] 何晓军[1] 王成[1] 

机构地区：[1]甘肃省疾病预防控制中心,兰州730020

出　　处：《中国卫生检验杂志》2012年第4期742-743,746,共3页Chinese Journal of Health Laboratory Technology

基　　金：甘肃省卫生行业科研计划项目(GSWST-2010-25)

摘　　要：目的:观察克雷伯菌在不同选择性培养基上的形态学特征。方法:根据克雷伯菌可发酵侧金盏花醇这一生化特性,对麦康凯培养基(MAC)加以改良,研制出改良麦康凯培养基(MACI)。选用生化特性近似的大肠菌群不同菌株接种到各选择性培养基上,以观察菌落形态特征。结果:克雷伯菌在MACI培养基上,形成独特的直径约为2 mm左右红色菌落。表明MACI具有高选择特异性和实际应用价值。结论:在克雷伯菌分离培养中推荐使用MACI培养基。Objective：To observe morphology characteristics of Klebsiella on the different culture medium.Methods：Based on Klebsiella rendering adonitol fermentation,MacConkey medium（MACI） was developed.Then the coliform with similar biochemical features was inoculated onto different selective medium to observe morphology characteristics of Klebsiella.Results：Klebsiella produced unique red bacterial colony with 2 mm of diameter on MACI,which indicated that MACI possessed high selectivity and value for identification of Klebsiella strains.Conclusion：MACI can be used as separating culture medium for Klebsiella.

关 键 词：克雷伯菌 选择性培养基 菌落特点 

分 类 号：R378.99[医药卫生—病原生物学]