慢性铅暴露对幼鼠海马CA_1区长时程增强的影响  

Effects of chronic exposure to lead on long-term potentiation in hippocampal CA_1 area of young rats in vivo

作　　者：刘君澜[1] 时利德[2] 万伯健[3] 李北利[3] 

机构地区：[1]广东医学院预防医学教研室,湛江市524023 [2]中国医科大学脑研究所神经生理室 [3]中国医科大学公共卫生学院卫生毒理室

出　　处：《中华预防医学杂志》2000年第1期34-36,共3页Chinese Journal of Preventive Medicine

摘　　要：目的　从突触功能可塑性的角度 ,探讨慢性铅暴露对脑学习记忆功能的影响。方法采用神经生理细胞外电位记录的方法 ,观察不同浓度铅暴露对大鼠海马CA1 区长时程增强 (LTP)在体诱导的影响。结果　大鼠血铅达 ( 3 2 8± 0 88) μmol L时 ,LTP发生率下降、增幅减小 ,甚至产生长时程抑制 ;染铅鼠的群体锋电位在高频刺激后 ,其平均增强率与脑铅呈负相关。结论　慢性铅暴露可损害海马CA1 区LTP的在体诱导和维持 ,其损害程度随染铅剂量增加而加重。Objective Tostudytheeffectsofchronicexposuretoleadonlearningandmemoryfunctionin youngrats,basedontheplasticityoftheirsynapsefunction .Methods Withmethodofextracellularglass microelectroderecording ,effectsofexposuretoleadatdifferentconcentrationsonlongtermpotentiation (LTP) inductioninhippocampalCA1 areaofyoungratsinvivowereobserved .Results IncidenceofLTPandmean amplitudeincreaseafterapplicationofhighfrequencystimulusdecreased ,asbloodleadinratsreached ( 3 2 8± 0 88) μmol L ,evencausinglongtermdepression .Totheexposedrats ,theirmeanincreaseratiointheir populationspikeamplitudesafterhigh frequencystimulationcorrelatedinverselywiththeirbrainleadload . Conclusion ChronicleadexposurecoulddamageinvivoLTPinductionandmaintenanceintheCA1 areain hippocampusofrats ,andtheseverityofdamageincreasedwiththeextentofexposuretolead .

关 键 词：铅暴露 海马CA1区长时程增强 记忆 幼鼠 

分 类 号：R99[医药卫生—毒理学]