一株抗辣椒疫病真菌的筛选及鉴定  

Screening and Identification of a Fungus Against Phytophthora Blight of Pepper

作　　者：王辉[1] 刘长远[1] 赵奎华[1] 孙军德[2] 

机构地区：[1]辽宁省农业科学院植物保护研究所,沈阳110161 [2]沈阳农业大学土地与环境学院,沈阳110866

出　　处：《吉林农业大学学报》2012年第2期152-156,共5页Journal of Jilin Agricultural University

基　　金：农业部公益性行业科研专项(201003004);辽宁省科技攻关项目(2010215003);辽宁省教育厅科研项目(L2010497)

摘　　要：从56份辣椒连作土壤中分离微生物782株,采用平板对峙法筛选出5株对辣椒疫病菌具有明显抑制作用的菌株,其中ASD菌株平板对峙试验结果表现最好,抑菌带宽11.7 mm,盆栽防效为75.12%,优于化学药剂代森锰锌和烯酰吗啉,有较好的应用开发前景。ASD菌株分生孢子头初为辐射形,后期为柱形;产孢结构双层,有明显的瓶梗及多育现象;分生孢子球形或近球形、壁滑,未见壳细胞;其rDNA-ITS序列与GenBank中已知序列比对,相似度97%,确定该菌株为黄柄曲霉(Aspergillus flavipes)。Seven hundred and eighty-two stains were isolated from 56 samples of continuous cropping soil for pepper.5 strains had obvious inhibitory effect on Phytophthora capsici by plate confrontation test,among which ASD showed the most significant inhibitory effect.The width of inhibition band was 11.7 mm.The results indicated that the control efficacy was 75.12% in the pot culture experiment.The control effect was better than dimethomorph and mancozeb,so it should be widely exploited and utilized in the future.The conidial head of ASD was radial on initial stage,and became cylindrical later.The sporing structure was double-layer,and had obvious phialides and proliferation.The conidium of ASD was globose,the wall was slithery,but the shell cell was not seen.Compared with the known-sequence in Genbank,the rDNA-ITS sequence of ASD was more than 97% similar to the species of Aspergillus.According to the above results,the strain was identified as Aspergillus flavipes.

关 键 词：辣椒疫病菌 黄柄曲霉 菌株鉴定 防病效果 

分 类 号：Q949.327.1[生物学—植物学] S436.418.1[农业科学—农业昆虫与害虫防治]