PMMA骨水泥微粒对成骨细胞增殖和功能的影响  

The effect of polymethyl methacrylate particles on the proliferation and function of osteoblast cell lines

作　　者：喻研[1] 吴萍[1] 朱亮[1] 孙黎[1] 徐瑶[1] 叶笃筠[1] 李钢[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,湖北武汉430030 [2]华中科技大学同济医学院附属梨园医院骨外科,湖北武汉430077

出　　处：《求医问药（下半月刊）》2012年第3期9-9,631,共2页Seek Medical and Ask The Medicine

基　　金：国家自然科学基金赞助(No.30700840)

摘　　要：目的:研究聚甲基丙烯酸甲酯(Polymethyl methacrylate,PMMA)骨水泥微粒对MC3T3-E1成骨细胞株(osteoblast,OB)增殖、分化和功能的影响,以初步探究人工关节无菌性松动的发生机制。方法:采用MTT法检测细胞增殖的变化,对硝基苯磷酸法和考马斯亮兰法作碱性磷酸酶(ALP)比活性测定。结果:PMMA微粒在各浓度(2.5mg/ml、5mg/ml、10mg/ml、15mg/ml、20mg/ml)时的OD值较未加PMMA颗粒的对照组均有增高。PMMA对细胞ALP比活性的影响呈双向改变,当PMMA浓度为0～25μg/ml时,ALP比活性随着PMMA浓度的升高而升高,当PMMA浓度为25～200μg/ml时,ALP比活性随着PMMA浓度的升高而降低,且呈浓度依赖性。结论:离体条件下PMMA对MC3T3-E1细胞的增殖有促进作用,对ALP活性具有双向影响。Aims：To study the effect of polymethyl methacrylate（PMMA） particles on the proliferation,differentiation and function of MC3T3-E1 osteoblast（OB） cell lines.Methods：Cell proliferation was detected with MTT assay.Alkaline phosphatase（ALP） activity of MC3T3-E1 cells was detected with kit.Result：When PMMA particle concentrations were 2.5 mg/ml、5 mg/ml、10 mg/ml、15 mg/ml、20 mg/ml,OD values were higher than that of the control group.PMMA had bipartite effect on the ALP activity,when PMMA concentration was 0～25？g/ml,ALP activity increased in according with the PMMA concentration,when PMMA concentration was 25—200？g/ml,ALP activity decreased in according with the PMMA concentration in a dose-depend manner.Conclusion：In vitro study,PMMA could significantly increase the cell proliferation while it had bipartite effect on the ALP activity in cultured MC3T3-E1 cell line.

关 键 词：骨水泥 成骨细胞 细胞增殖 碱性磷酸酶 

分 类 号：R687.3[医药卫生—骨科学]