镉对人胚肾细胞中经典瞬时受体电位通道mRNA表达影响  

Effects of cadmium on expressions of TRPC mRNA in human embryo kidney cells

作　　者：余少珍[1] 李文军[1] 张敏红[1] 薛永[1] 邹飞[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院职业卫生与职业医学系,广东广州510515

出　　处：《中国职业医学》2012年第2期98-101,共4页China Occupational Medicine

基　　金：国家自然科学基金资助项目(30971193)

摘　　要：目的观察不同质量浓度的氯化镉(CdCl2)染毒后人胚肾细胞(HEK 293T细胞)经典瞬时受体电位通道(TRPC)6个亚型mRNA表达水平的改变,在mRNA水平上探讨镉对肾细胞钙通道的影响。方法 HEK 293T细胞分别暴露于7.5、15.0、30.0、45.0和60.0μmol/L的CdCl28 h后,应用四甲基偶氮唑蓝(MTT)比色法测定细胞增殖活力;实时荧光定量聚合酶链反应法检测0、15.0和30.0μmol/L CdCl2作用8 h和30.0μmol/L CdCl2染毒4 h后TRPC 6个亚型mRNA表达水平。结果以对照组(CdCl20μmol/L)HEK 293T细胞存活率为100%,各不同质量浓度的染毒组细胞存活率分别为79.40%、77.29%、72.26%、70.25%、60.80%,与对照组比较,差异有统计学意义(P<0.05或P<0.01)。经CdCl2染毒后,各染毒组HEK 293T细胞的TRPC 6个亚型mRNA相对表达量均低于对照组(P<0.05或P<0.01)。结论 CdCl2可能引起HEK 293T细胞的TRPC基因mRNA的表达水平发生改变。Objective To study the effects of cadmium chloride （ CdC12 ） on the expressions of transient receptor potential canonical（TRPC） mRNA in human embryo kidney ceils （HEK 293T）, and to discuss the effects of CdC12 on the calcium chan- nel in HEK 293T cells. Methods HEK 293T cells were exposed to CdC12 for 8 hours at the mass concentrations of 7.5, 15.0, 30. 0, 45.0 and 60. 0 μmol/L respectively. The cell viability was measured with MTF colorimetric assay. The expressions of TR- PC1, TRPC3, TRPCA, TRPC5, TRPC6 and TRPC7 mRNA were determined in HEK 239T cells after exposure to 0, 15.0, 30. 0 μmol/L CdC12 for 8 hours and 30. 0 μmol/L CdC12 for 4 hours by quantitative RT-PCR. Results Compared with the control group （ CdC12 0 μmol/L）, the survival rate of HEK 293T cells were 79.40%, 77.29%, 72.26%, 70.25%, 60. 80% （ P 〈 0. 05 or P 〈 0. 01 ） respectively at the mass concentrations of 7. 5, 15.0, 30. 0, 45.0 and 60. 0 μmol/L CdCl2 after 8 h expo- sure. The mRNA expressions of TRPC （TRPC1, TRPC3, TRPCA, TRPC5, TRPC6, TRPC7 ） were decreased after treated with CdC12 （ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion CdC12 may be able to change the expressions of TRPC mRNA in HEK 293T cells.

关 键 词：经典瞬时受体电位通道 人胚肾细胞 镉 

分 类 号：R135.1[医药卫生—劳动卫生]