SPE-HPLC-ELSD法测定三七药材中单糖和二糖的含量  

Determination of Monosaccharide and Disaccharide in Panax notoginseng by HPLC with SPE and ELSD

作　　者：高阳[1,2] 牛涛 陈虹 徐波 孔令义[1] 

机构地区：[1]中国药科大学天然药物化学教研室,江苏南京210009 [2]天津天士力现代中药资源股份有限公司,天津300402

出　　处：《现代中药研究与实践》2012年第2期29-31,共3页Research and Practice on Chinese Medicines

摘　　要：目的建立高效液相色谱-蒸发光散射检测法,同时测定三七药材中单糖和二糖的含量。方法 HPLC采用Thermo Hypersil GOLDAmino氨基柱（250×4.6 mm）,流动相为乙腈-水（75：25）,流速0.8mL·min^-1,ELSD漂移管温度75℃,载气为空气,载气流速2.5 L ·min^-1。结果在该条件下三七药材中的单糖和二糖组分能够得到较好的分离,线性关系良好（相关系数R^2〉0.99）,回收率在98.23%-101.46%之间,且相对标准偏差小于3%。结论该方法简便、准确、灵敏、稳定,适合于三七药材中单糖和二糖的含量测定。Objective An HPLC-ELSD method was established for the simultaneous quantitative analysis of glucose,fructose,sucrose from Panax notoginseng.Methods The chromatographic separation was achieved on an amino column using a mobile phase composed of a mixture of acetonitrile and water（75：25） at a flow rate of 0.8 mL/min by gradient elution.The ELSD drift tube temperature was set at 75 ℃ and the carrying gas was air.Results Under the above conditions,monosaccharides and disaccharides from Panax notoginseng could be well separated,and the spiked recoveries were 98.23%-101.46% with a RSD less than 3%（n = 3）.Conclusion This method was accurate,rapid,simple and reproducible,and thus it would be suitable for the determination of monosaccharides,disaccharides from Panax notoginseng.

关 键 词：三七 高效液相色谱-蒸发光散射检测 单糖 二糖 

分 类 号：R927.2[医药卫生—药学]