抑制p38MAPK活化对脓毒症大鼠肺部及血管组织中iNOS及NO合成的影响  

Effects of p38 Mitogen Activated Protein Kinase Pathway on Synthesis of iNOS and NO in Pulmonary and Vascular Tissues of Infectious Shock Rats

作　　者：王松柏[1] 翟秀珍[2] 曹怀宇[1] 张艳丽[1] 

机构地区：[1]中国人民解放军第251医院麻醉科,河北张家口075000 [2]河北北方学院附属第一医院神经内科,张家口075000

出　　处：《河北北方学院学报（自然科学版）》2012年第2期64-67,共4页Journal of Hebei North University:Natural Science Edition

摘　　要：目的通过抑制MAPK通路活化,观察其对脓毒症大鼠组织和血清诱导型一氧化氮合成酶(iN-OS)及一氧化氮(NO)合成的影响以及循环变化.方法大鼠脓毒症模型采用经典的盲肠结扎穿孔术(cecalligation puncture,CLP),将SD大鼠随机分为正常对照组、假手术组、CLP脓毒症组和SB203580治疗组.采集组织和血清并测定组织iNOSmRNA表达水平,NO检测试剂盒(酶法)检测组织和血清NO含量;同时分别监测上述各时间点的脉搏和平均动脉压.结果与正常组大鼠相比,CLP后各时间点脓毒症大鼠肺、血管和血清iNOS mRNA、NO含量均升高明显;而MAP下降明显、脉搏明显升高(P<0.05,P<0.01).治疗组与CLP组相应时间点相比,iNOSmRNA表达在肺和血管多个时间点降低;而肺和血管及血清NO含量下降;血压和脉搏均显著好转(P<0.05,P<0.01).相关分析显示:血管和肺组织iNOSmRNA与NO的相关系数分别是0.75和0.74;肺、血管组织iNOS mRNA与血清NO的相关系数分别是0.69和0.65(P<0.05),MAP与血管、肺和血清NO的相关系数分别是-0.85、-0.86和-0.90(P<0.05).结论抑制MAPK通路活化可抑制脓毒症大鼠血浆和组织iNOSmRNA及NO合成,并改善循环功能.Objective To investigate the effects of p38 mitogen activated protein kinase on synthesis of inducible nitric oxide synthase（iNOS）and nitric oxide（NO）in infectious shock rats.Methods SD rats were randomly divided into normal control,sham operation,cecal ligation puncture（CLP）,and inhibitor of p38MAPK（SB203580）treatment groups Rats in.CLP and treatment groups were established into sepsis model induced by CLP,and at 2,6,24 h after CLP,the rats were sacrificed.The vascular,pulmonary tissues and serum samples of all rats were harvested to determine iNOS mRNA expression levels by reverse transcription polymerase chain reaction and NO content respectively.Meanwhile,the mean arterial pressure（MAP）and pulse were monitored.Results Compared with that of normal control,iNOS mRNA and NO levels in vascular,pulmonary tissues and serum at 2,6 and 24 h following CLP significantly increased respectively,and MAP fell notably and pulse increased（P0.05 or 0.01）.SB203580 treatment reduced iNOS mRNA and NO levels markedly at 2 or 6 h after CLP,meanwhile,MAP and pulse deteriorated by sepsis were also ameliorated（P0.05 or 0.01）.Conclusion Inhibiting the activation of MAPK pathway can decrease tissues and serum iNOS mRNA and NO expressions in infectious shock rats and can ameliorate the circulatory function deteriorated by sepsis.

关 键 词：大鼠 脓毒症 MAPK信号通路 NO INOS 

分 类 号：R631[医药卫生—外科学]