慢性间隙性缺氧中氧化应激对血管内皮的影响  

The efecct of oxidative stress on aorta endothelial cell in chronic intermittent hypoxia animal model

作　　者：李洁[1] 李兵[1] 杨湘[1] 莫海兰[1] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉科,重庆400016

出　　处：《临床耳鼻咽喉头颈外科杂志》2012年第9期411-413,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：重庆卫生局科研项目(No:2008-2-38)

摘　　要：目的:制作可应用于常压慢性间隙性缺氧(CIH)研究的动物模型,探讨CIH大鼠模型中氧化应激指标与主动脉内皮细胞损伤的关系。方法:24只SD大鼠随机分为空白对照组(正常饲养4周,UC组)、CIH组(CIH 4周)和复氧组(CIH 4周后复氧4周,RH组),每组8只。实验结束后测定大鼠血清中氧化应激指标丙二醛(MDA)、超氧歧化物(SOD)、NO的水平。处死大鼠取出腹主动脉,光镜下观察主动脉内皮细胞形态及内皮型一氧化氮合酶(eNOS)的表达情况。结果:CIH组SOD、NO较UC组均明显降低,而MDA显著升高(均P<0.05);大鼠腹主动脉内皮形态改变,eNOS表达减弱,明显低于UC组。RH组、UC组SOD、NO及MDA比较,差异无统计学意义(均P>0.05);2组大鼠腹主动脉内皮形态均未见明显改变,eNOS的表达比较差异无统计学意义(均P>0.05)。结论:大鼠CIH模型中氧化应激参与了主动脉的病变过程。Objective：To make a chronic intermittent hypoxia（CIH） animal model and explore the relationships of oxidative stress and the injury of aorta endothelial cell on the CIH rat model.Method：Twenty-four sex maturation rat were randomly Divided into unhandled control group（normal feeding for 4 weeks,UC group）,CIH group（CIH for 4 weeks）,reoxygenation group（normal feeding for 4 weeks after CIH for 4 weeks,RH group）.Determined the activity of superoxide dismutase（SOD）,malondialdehyde（MDA） and nitric oxide（NO） in blood serum of all groups.Dislodged rat＇s aorta,observed the appearance of aorta endothelial cell and the expression of endothelial nitric oxide synthase（eNOS）.Result：Compared with UC group,in CIH group the activities of SOD and NO were significantly lower,but MDA significantly higher（all of P0.05 respectively）,the appearance of aorta endothelial cell changed,the expressions of eNOS lower.There were not significantly change in UC group and RH group（P0.05）.Conclusion：Oxidative stress played a role in the injury of aorta endothelial cell on ob chronic intermittent hypoxia rat modle.

关 键 词：慢性间隙性缺氧 氧化应激 主动脉内皮细胞 

分 类 号：R766[医药卫生—耳鼻咽喉科] R767[医药卫生—临床医学]