三白草中三白草酮提取工艺优选  

Optimization of Extraction Technology for Sauchinone from Saururus chinensis

作　　者：薛平[1] 徐春蕾[1] 李祥[1] 陈建伟[1] 陈勇[1] 陈瀚[1] 

机构地区：[1]南京中医药大学,南京210046

出　　处：《中国实验方剂学杂志》2012年第9期43-45,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏高校优势学科建设工程项目(ysxk-2010);2011年江苏省普通高校研究生科技创新计划(794);2011年度南京中医药大学校青年自然科学基金项目(11XZR11)

摘　　要：目的:优选三白草中三白草酮的提取工艺参数。方法:采用HPLC测定三白草酮提出量,并以此为指标,正交试验法对粉碎度、乙醇体积分数、料液比、提取时间、浸泡时间5个因素进行考察,优选最佳提取工艺。结果:最佳提取条件为药材过10目筛,95%乙醇提取2次,第1次加14倍量乙醇浸泡2 h,提取2 h;第2次加10倍量乙醇提取1 h。在此条件下,三白草酮平均提取得率0.21%,提取转移率为96%。结论:优选的提取工艺稳定、合理,可用于三白草中三白草酮的提取。Objective： To optimize extraction technology parameters of sauchinone from Saururus chinensis.Method：With the content of sauchinone as index,it was determined by HPLC.Optimum extraction technology was optimized by orthogonal test with grinding degree,the concentration of ethanol,ratio of solid-liquid,extraction time and soaking times as factors.Result： Optimum extraction conditions were：drugs passed 10 mesh sieve,extracted 2 times with 95% ethanol,14 times the amount of 95% ethanol was added for extracting 2 hours firstly,extracted 1 h with 10 times the amount of 95% ethanol in second time.In this conditions,average extraction yield of sauchinone was 0.21%,extraction transfer ratio was 96%.Conclusion： Optimized extraction technology was stable,reasonable and could be used for extraction of sauchinone from S.chinensis.

关 键 词：三白草 三白草酮 正交试验 提取工艺 

分 类 号：R283.6[医药卫生—中药学]