元胡止痛软胶囊中延胡索提取工艺优选  

Optimization of Extraction Process for Corydalis yanhusuo from Yuanhu Zhitong Soft Capsule

作　　者：杜茂波[1] 刘淑芝[1] 刘树扬[1] 葛克亚[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2012年第9期50-52,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院中药研究所自主选题项目(ZZ03048)

摘　　要：目的:优化元胡止痛软胶囊中延胡索的提取工艺。方法:以延胡索乙素含量为指标,HPLC测定其含量,选用L9(34)正交试验设计,考察乙醇体积分数、加醇倍数、提取次数、提取时间等影响因素。结果:优选的提取工艺为6倍量70%乙醇提取3次,每次1.5 h。3批验证试验RSD 2.69%。结论:优选的提取工艺稳定、可行。Objective：To optimize extraction process of Corydalis yanhusuo from Yuanhu Zhitong soft capsule.Method： With the content of tetrahydropalmatine as an indictor by HPLC,L9（34） orthogonal test was used to investigate influencing factors,such as the concentration of ethanol,the amount of ethanol,extration times,extraction time and so on.Result：Optimized extraction technology was：extracted 3 times with 6 times the amount of 70% ethanol,each time 1.5 h.RSD of three batches of validation tests was 2.60%.Conclusion：This optimized extraction pocess of C.yanhusuo from Yuanhu Zhitong soft capsule was stable and available.

关 键 词：元胡止痛软胶囊 延胡索乙素 提取 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]