HPLC测定广西产匙羹藤叶中牛弥菜醇A含量  

Determination of Conduritol A in Leaf of Gymnema sylvestre by HPLC

作　　者：丘琴[1,2] 甄汉深[2] 黄小秋[2] 黄培倩[2] 石琳[2] 

机构地区：[1]成都中医药大学,成都611137 [2]广西中医学院,南宁530001

出　　处：《中国实验方剂学杂志》2012年第9期136-138,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西自然科学基金项目(桂科自0229044);广西教育厅科研项目(桂教科2001);广西中医学院重点科研项目(ZD2004010)

摘　　要：目的:对广西产匙羹藤叶中牛弥菜醇A的含量进行测定,为科学评价及有效控制其质量提供依据。方法:采用高效液相色谱法,以氨基柱(4.6 mm×250 mm,5μm)为色谱柱,流动相乙腈-水(87∶13),流速1.0 mL.min-1,检测波长207nm。结果:牛弥菜醇A在2.4～12μg与峰面积成良好的线性关系(r=0.999 7),平均回收率为101.39%,RSD 0.74%。结论:所建立的方法准确,快速,灵敏度高,重复性好,可作为广西产匙羹藤叶的质量控制方法。Objective： To determine the content of conduritol A in the leaf of Gymnema sylvestre.Method： HPLC was adopted,the separation was performed on a Thermo NH2 column（4.6 mm×250 mm,5 μm）,acetonitrile-water（87∶ 13） was used as the mobile phase,the flow rate was 1.0 mL·min-1,the detection wavelength was set at 207 nm.Result：The liner range of conduritol A was 2.4-12 μg（r=0.999 7）,the average recovery rate was 101.39%,RSD was 0.74%.Conclusion： The method was accurate,sensitive,quick and simple.It can be used as the quality control method of the leaf of G.sylvestre.

关 键 词：匙羹藤叶 牛弥菜醇A 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]