决明子中化学对照品红镰霉素龙胆二糖苷稳定性  

Stability of Chemical Reference Rubrofusarin-6-O-β-gentiobioside of Semen Cassiae

作　　者：姜艳艳[1] 徐冬艳[1] 王月明[1] 刘慧[1] 刘斌[1] 石任兵[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2012年第9期142-145,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"十一五"科技支撑计划项目(2006BAI08B03-04);北京中医药大学科研创新团队项目(2011-CXTD-12)

摘　　要：目的:研究决明子中化学对照品红镰霉素龙胆二糖苷的稳定性。方法:将红镰霉素-6-O-β-龙胆二糖苷分别置于不同温度、湿度及不同溶剂环境下处理,采用高效液相色谱法测定含量,计算剩余率。结果:温度对红镰霉素龙胆二糖苷的稳定性影响较大,湿度影响次之,温度、湿度越高,稳定性越差;红镰霉素龙胆二糖苷在甲醇、乙醇中较为稳定,在水、0.1%盐酸溶液稳定性次之,在0.1%氨水溶液中稳定性最差。结论:红镰霉素龙胆二糖苷应置于干燥器中低温或室温密封保存;红镰霉素龙胆二糖苷对照品溶液应选择甲醇和乙醇为溶剂,低温保存。Objective： To study the stability of rubrofusarin-6-O-β-gentiobioside of Semen Cassiae.Method： Rubrofusarin-6-O-β-gentiobioside was processed under different temperature,humidity and solvent respectively.The content was determined by HPLC and the remaining percentages of rubrofusarin-6-O-β-gentiobioside were calculated.Result： The stability of rubrofusarin-6-O-β-gentiobioside was influenced mostly by temperature,and secondly by humidity.The stability of rubrofusarin-6-O-β-gentiobioside reduced along with the temperature rising and the humidity increasing;rubrofusarin-6-O-β-gentiobioside was much more stable in methanol and ethanol than in water and 0.1% hydrochloric acid aqueous solution,and rubrofusarin-6-O-β-gentiobioside transferred to other substance in 0.1% ammonia aqueous solution.Conclusion： Rubrofusarin-6-O-β-gentiobioside should be preserved under low or room temperature in dry condition.Methanol or ethanol should be selected as solvent of rubrofusarin-6-O-β-gentiobioside solution,preserved under low temperature.

关 键 词：决明子 红镰霉素龙胆二糖苷 稳定性 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]