乌骨藤中白桦酯酸的提取分离及抗肿瘤活性  

Study on Extraction,Isolation and Antitumor Activity of Betulinic Acid from Caulis Marsdeniae tenocissimae

作　　者：倪冲[1] 裴志东[1] 张镓小[1] 李春雪[1] 张慧[1] 康廷国[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国实验方剂学杂志》2012年第9期172-175,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国博士后科学基金特别项目(200902550);辽宁省教育厅基金资助项目(2009T066);大连科技局青年基金项目(2009J22DW040)

摘　　要：目的:对乌骨藤中白桦酯酸进行提取分离,并探讨其抗肿瘤活性。方法:采用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法及半制备高效液相色谱法,对白桦酯酸进行提取分离,并采用结晶紫法,以细胞死亡率为检测指标,观察白桦酯酸在不同质量浓度1～500 mg·L-1情况下,对细胞密度为1×104/孔的4种肿瘤细胞分别作用12,24,36,48 h的生长抑制作用。结果:白桦酯酸对人宫颈癌细胞HeLa、人胃癌细胞MGC、人乳腺癌细胞MCF-7具有很好的增殖抑制作用,并呈剂量依赖效应,在质量浓度为500 mg·L-1作用48 h时,对Hela细胞及MGC细胞的死亡率分别为77.9%,81.7%,质量浓度为100 mg·L-1作用48 h时,对MCF-7的细胞死亡率为74.1%,但对人乳腺导管癌细胞(ZR株)作用不显著。结论:白桦酯酸对HeLa,MGC,MCF-7肿瘤细胞具有抗肿瘤活性,对ZR株肿瘤细胞作用不明显。Objective： Extraction and isolation of betulinic acid from Marsdenia tenacissima and investigate the anti-tumor activity were investigated.Method： By using silica gel column chromatography,Sephadex LH-20 chromatography and semi-preparative reversed phase liquid chromatograph,extraction and isolation of betulinic acid,and MTT assay was applied in demonstrating effect on antitumor effect in four tumor cells with different concentrations and at different action time.Result：The growth of cervical cancer cells HeLa,gastric cancer cells MGC,breast cancer MCF-7 was significantly inhibited by betulinic acid with a manner of dose-dependence.After 48 h,the cell death rate of HeLa and MGC was 77.9%,81.7%,respectively.When the concentration of the sample was 500 mg·L-1,the cell death rate of MCF-7 was 74.1%,when the concentration of the sample was 100 mg·L-1,the effect on ducal carcinoma cells was not significant.Conclusion： Betulinic acid has an anti-tumor activity on HeLa,MGC,MCF-7.

关 键 词：白桦酯酸 乌骨藤 提取分离 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]