火麻仁油对衰老模型小鼠皮肤相关指标的影响  

The Influence of the Hemp Seed Oil on the Related Parameters of the Skin in Aging Mice

作　　者：李寒冰[1] 马永洁[1] 苗静静[1] 张娟[1] 任汉阳[1] 李根林[1] 

机构地区：[1]河南中医学院药学院,郑州450008

出　　处：《中国实验方剂学杂志》2012年第9期201-205,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关重点项目(102102310320);河南省自然科学基金项目(2003-11044900);河南中医学院博士科研基金(BSJJ2009-09)

摘　　要：目的:观察火麻仁油对衰老模型小鼠皮肤相关指标的影响。方法:采用D-半乳糖颈背部皮下注射(sc)复制小鼠亚急性衰老模型,造模1 h后各给药组灌胃(ig)给与不同剂量试验药物,共42 d,同时分别观察火麻仁油高、中、低剂量(12.0,6.0,3.0 mL·kg-1)对衰老模型小鼠皮肤匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、脂褐质(Lpf)、羟脯氨酸(Hyp)、皮肤水分含量的影响;对比各组实验动物相同部位皮肤的组织病理学形态,表皮及真皮厚度,真皮胶原纤维排列松密情况、弹力纤维面积及形态的差别变化。结果:火麻仁油高剂量组MDA,Lpf平均含量分别为7.13 nmol·mg-1和0.83μg·g-1,中剂量组MDA平均含量为7.29 nmol·mg-1,与模型组相比均有显著下降(P<0.05);高、中剂量组SOD活性平均值分别为21.95 U·mg-1和21.45 U·mg-1;而Hyp含量平均值分别为8.31μg·mg-1和7.92μg·mg-1,它们与模型组相比均有显著提高(P<0.01,P<0.05);高、中剂量组也显著升高皮肤水分的水平(P<0.01～P<0.05),低剂量对上述指标的影响无统计学意义。火麻仁油能改善和促进衰老模型小鼠毛发的生长,病理观察真皮厚度和毛囊数均有增加;胶原纤维、弹力纤维的数量和形态均有明显的改善。结论:火麻仁油能够对模型小鼠发挥抗皮肤衰老和抗氧化作用。Objective： To observe the influence of the hemp seed oil on the related parameters of skin in the aging mice,and study the anti-aging function of the fructus cannabis.Method： D-galactose was given by hypodermic injection to copy the subacute aging model of the mice.1 h later,corresponding drugs were given to their groups for 42 days.The influence of hemp seed oil of high,medium,and low doses（12.0,6.0,3.0 mL·kg-1） on superoxide dismutase（SOD）,malondiadehyde（MDA）,lipofuscin（Lpf）,hydroxyproline（Hyp） and skin moisture content were observed,respectively.Histopathological observation of mice skin including the epidermal and dermal thickness of the mice skin at the same part of the body was observed.The difference between dermal collagen fibers arrays of different experimental groups was compared,and the area and morphological changes of the elastic fibers were examined.Result：The average contents of MDA and Lpf in skin homogenates of large dose group were 7.13 nmol·mg-1and 0.83 μg·g-1,the average contents of MDA in skin homogenates of medium dose group were 7.29 nmol·mg-1,all of these indexes were decreased significantly compared with which of model group（P0.05）.The average activitis of SOD in skin homogenates of large medium dose groups were 21.95 U·mg-1 and 21.45 U·mg-1,the Hyp contents of them were 8.31 μg·mg-1 and 7.92 μg·mg-1,all of these indexes were increased significantly compared with which of model group（P0.01~P0.05）.The large and medium doses could greatly increase moisture content in the mice skin（P0.01~P0.05）.The small dose showed some influence on the above indexes,but the effect was not significant.The hemp seed oil could improve the fur growing condition of the model mice.The dermal thickness and the number of hair follicles of the model mice were considerably increased.The texture of dermal collagen fibers and elastic fibers were obviously improved.Conclusion： The hemp seed oil can exert its function of anti-skin-aging and antioxidation.

关 键 词：火麻仁油 抗皮肤衰老 抗氧化 形态学 

分 类 号：R285.5[医药卫生—中药学]