白藜芦醇联合姜黄素对SMMC-7721肝癌细胞作用  

Combination of Resveratrol and Curcumin Inhibits Proliferation and Induces Apoptosis in Human Hepatocarcinoma SMMC-7721 Cells

作　　者：杜琴[1] 胡兵[1] 沈克平[1] 邓珊[1] 

机构地区：[1]上海中医药大学附属龙华医院肿瘤科中医肿瘤研究所,上海200032

出　　处：《中国实验方剂学杂志》2012年第9期262-266,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市基础研究重点项目(09JC1413600);龙华医院国家中医临床研究基地"龙医团队;龙医学者"项目(LYTD-04)

摘　　要：目的:观察白藜芦醇联合姜黄素对体外人肝癌细胞SMMC-7721增殖和凋亡的影响及相关信号通路。方法:不同浓度白藜芦醇、姜黄素及两药联合干预SMMC-7721细胞,MTT法检测细胞增殖,流式细胞术检测细胞凋亡、Hoechst 33258染色检测细胞凋亡形态变化,比色法检测半胱氨酸天冬氨酸蛋白酶(caspase)-3,caspase-8,caspase-9酶活性,Western blot法检测半胱氨酸天冬氨酸蛋白酶切割底物(PARP)。结果:与对照组相比,白藜芦醇、姜黄素单独或联合作用SMMC-7721细胞均可抑制SMMC-7721细胞增殖,两药联合后抑制作用更显著。白藜芦醇、姜黄素联合较单独用药可增强SMMC-7721细胞凋亡,呈现凋亡形态改变,白藜芦醇、姜黄素及联合组细胞凋亡率分别为(17.39±1.41)%,(14.96±2.23)%,(25.36±2.68)%;同时提高SMMC-7721细胞caspase-3,caspase-8及caspase-9活性,促使PARP蛋白剪辑。结论:白藜芦醇、姜黄素联合使用可增强对人肝癌细胞SMMC-7721的抗癌作用,并可能与caspase-8,caspase-9/caspase-3/PARP信号通路介导细胞凋亡相关。Objective： To observe the combinational effects of resveratrol and curcumin on cell proliferation,apoptosis and the possible mechanisms in human hepatocarcinoma SMMC-7721 cells in vitro.Method： SMMC-7721 cells were treated with resveratrol or curcumin or both.Cell proliferation was detected by MTT assay.Cell apoptosis was detected by flow cytometry,apoptotic morphology was visualized by hoechst 33258 staining.Caspase-3,caspase-8 and caspase-9 activities were detected by colorimetric assay,and cleaved poly（ADP-ribose） polymerase（PARP） was detected by Western blot.Result： Compared with the control,resveratrol and curcumin significantly inhibited the proliferation of SMMC-7721 cells.The combination of resveratrol and curcumin was found to be more effective in inhibiting growth（P0.01）,and inducing apoptosis in SMMC-7721 as indicated by apoptotic morphological change and PI/Annexin V-FITC staining.The apoptosis rate of resveratrol,curcumin and combination group was（17.39±1.41）%,（14.96±2.23）%,and（25.36±2.68）% respectively.In addition,caspase-3,caspase-8 and caspase-9 were significantly activated by combinational treatment of resveratrol and curcumin,accompanied by increased PARP cleavage,and compared with either agent alone.Conclusion： Combination of resveratrol and curcumin may elicit synergistic anti-cancer effects in human hepatocarcinoma SMMC-7721 cells,and associated with caspase-8,-9/caspase-3/PARP mediated apoptosis.

关 键 词：肝癌 白藜芦醇 姜黄素 细胞凋亡 半胱氨酸天冬氨酸蛋白酶 切割底物 

分 类 号：R285[医药卫生—中药学]