蚧虫初孵若虫采集、保存和玻片标本制作  

Specimen Preservation and Slide Preparation for Newly Hatched Larvae Nymph of Scale Insects

作　　者：赵杰军[1] 王自力[1] 陈晓鸣[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,昆明650224

出　　处：《东北林业大学学报》2012年第5期128-130,共3页Journal of Northeast Forestry University

基　　金：国家林业局林业公益性行业科研专项经费项目(201204602);"十二五"国家科技计划课题(2011BAD33B02);国家自然科学基金项目(30771736);中国林科院中央级公益性科研院所基本科研业务费专项资金项目(riricaf201004M)

摘　　要：蚧虫初孵若虫用FAA固定液固定有利于玻片制作和长期保存。在虫体软化、脱水、染色和清洗过程中,使用自制转移虫袋,使得虫体转移更为简易,并可以进行批量处理。用丁香油替代二甲苯、Euparal替代中性树脂或加拿大树胶,使制作过程更具有安全性。Methods of fixation and slide preparation for newly hatched larvae nymph of scale insects are introduced. FAA fixation is favorable to long-term preservation and slide preparation for newly hatched larvae nymph of scale insets. Self-made bags were used to transfer the insects during maceration, dehydration, coloration, and cleaning, which made larvae transferring easier and mass production possible. The process of slide preparation would be more safety by replacing Canada balsam or neutral resins by euparal and xylene by clove oil, respectively.

关 键 词：蚧虫 初孵幼虫 玻片制作 微小昆虫 

分 类 号：Q964[生物学—昆虫学]