水网藻种植水对铜绿微囊藻生长的抑制作用研究  

Inhibitory Effects of Liquor Cultured with Hydrodictyon reticulatum on the Growth of Microcystis aeruginosa

作　　者：傅海燕[1] 柴天[1] 赵坤[1,2,3] 刘智峰[4] 张明真[1,2] 侯明[1,2] 许鹏成[1,2] 

机构地区：[1]厦门理工学院环境工程系,厦门361024 [2]华北电力大学能源与环境研究院,北京102206 [3]中国水电顾问集团中南勘测设计研究院,长沙410014 [4]湖南大学环境科学与工程学院,长沙410082

出　　处：《环境科学》2012年第5期1564-1569,共6页Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2009ZX07104-004);国家自然科学基金项目(51109181);厦门市科技计划项目(3502Z20093040);福建省教育厅项目(JA11236)

摘　　要：研究了不同浓度水网藻种植水对铜绿微囊藻生长的抑制作用以及对叶绿素a和抗氧化酶活性(SOD、CAT、POD)的影响.结果表明,水网藻种植水对铜绿微囊藻的抑制效果明显,20%～80%浓度的种植水第8 d的平均抑藻率达到98.9%,藻细胞几乎全部死亡.在种植水的作用下藻细胞叶绿素a含量迅速降低,叶绿素a遭到破坏.实验前2 d由于受到活性氧的刺激铜绿微囊藻抗氧化酶活性增加,且都高于对照组,随着抗氧化酶活性达到极限,活性氧开始积累并破坏细胞的正常代谢,抗氧化酶活性迅速降低,80%浓度的实验组至第8 d时SOD、CAT、POD活性分别仅为31、6、5 U.mg-1.水网藻种植水经过高温处理后对铜绿微囊藻基本没有抑制作用,说明种植水中的抑藻物质具有热不稳定性.The inhibitory effects of aquatic filtrate from Hydrodictyon reticulatum culture on the growth of Microcystis aeruginosa and the influences on its chlorophyll a content and activities of antioxidant enzymes（SOD,CAT and POD） were studied.The results showed that liquor cultured with H.reticulatum could inhibit the growth of M.aeruginosa markedly,displayed that the average inhibition ratio was 98.9% on the 8th day with 20%-80% liquor cultured with H.reticulatum which meant algal cell almost died.The chlorophyll a content of M.aeruginosa decreased by liquor cultured with H.reticulatum.Due to stimulative function of active oxygen,the activities of antioxidant enzymes of M.aeruginosa increased at the first 2 days and they were higher than that of the control group.With the limit of activities of antioxidant enzymes coming,active oxygen began to accumulate which damaged normal cellular metabolism and decreased the activities of antioxidant enzymes rapidly.The activities of SOD,CAT and POD were only 31,6,5 U·mg-1 respectively on the 8th day with 80% liquor cultured with H.reticulatum.The inhibitory effects of liquor cultured with H.reticulatum after high-temperature treatment on M.aeruginosa were unconspicuous,which demonstrated that liquor cultured with H.reticulatum was thermally-instable.

关 键 词：水网藻 铜绿微囊藻 化感作用 叶绿素A 抗氧化酶活性 降解特性 

分 类 号：X52[环境科学与工程—环境工程] X171