转il-4基因甘蓝后代的遗传及表现分析  

Study on Analysis of Genetic and Performance of Offspring Transforming il-4 Gene into Brassica oleracea

作　　者：张玮滢[1] 于丽杰[1] 

机构地区：[1]黑龙江省普通高等学校植物生物学重点实验室,哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025

出　　处：《北方园艺》2012年第8期133-137,共5页Northern Horticulture

基　　金：黑龙江省普通高等学校骨干教师创新能力资助项目;黑龙江省自然科学基金资助项目(C0014);哈尔滨市学科后备带头人基金资助项目(0071007002)

摘　　要：利用农杆菌介导法将il-4基因转入甘蓝品种"中甘11",用已转化得到的T3、T4代甘蓝品种为试验材料,通过PCR扩增、PCR-Southern杂交、蛋白质浓度测定和ELISA技术,对il-4基因转化甘蓝的后代进行了检测,并对il-4基因在T3、T4代甘蓝中的传递规律以及后代甘蓝植株的田间表现进行了调查。结果表明:T3、T4代转il-4基因甘蓝阳性植株数较少,甘蓝中il-4基因出现性状分离。同时,田间调查表明,相对于对照组(非转基因)植株,转il-4基因阳性植株的田间抗病性下降,植株高度降低,结实率降低,单株结籽数减少,生长势等农艺性状降低。The il-4gene was transformed into Brassica oleracea cv.‘Zhonggan 11’ by using Agrobacterium-mediated method.The obtained T 3 and T 4generation materials were detected by using PCR amplification,PCR-southern blotting,protein concentration determination and ELISA.Meanwhile the genetic rule of il-4gene and the phenotypes of progenies were investigated.The results showed that a few positive transgenic cabbage plants were detected in T3 and T4 generation.The genetic rule of il-4gene in transgenic cabbages didn’ t according with Mendel law.Meanwhile,the field survey showed that the disease resistance,height,seed setting rate,growth potential decreased comparing to the control.

关 键 词：甘蓝 白细胞介素-4 分子检测 田间分析 

分 类 号：S635.603.6[农业科学—蔬菜学]