肠道病毒71型抵抗I型干扰素诱导的抗病毒作用  

Human enterovirus 71 （EV71） resisted type I interferon induced antiviral effect

作　　者：崔雨[1] 宋娟[2] 宋芹芹[2] 孙鹏[2] 甘星[2] 李公启[2] 王克霞[1] 韩俊[2] 

机构地区：[1]安徽理工大学医学院病原微生物教研室,淮南232001 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室

出　　处：《中华实验和临床病毒学杂志》2012年第2期102-104,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：传染病重大专项（2009ZX10004-101、2008ZX10004-002）和重点实验室发展基金（2008SKLID102、2011SKLID104）

摘　　要：目的研究肠道病毒71型（EV71）抵抗I型干扰素（interferon，IFN）诱导的抗病毒作用。方法将1000U／ml的I型干扰素（α，β）加入HeLa细胞后，去除上清中的干扰素，感染带有GFP的重组单纯疱疹病毒（HSV-1）和EV71，观察GFP的表达和PCR检测HSV-1核酸，判断I型干扰素对HSV，1的作用。通过RT—PCR方法检测EV712A基因的表达从而判断EV71病毒的复制能力。结果I型干扰素（α，β）诱导HeLa细胞产生抗病毒蛋白而有效地抑制重组HSV-1GFP的表达和核酸扩增。而EV712A的RT—PCR结果证实EV71可在I型干扰素（α，β）作用后的HeLa细胞中有效增殖。结论I型干扰素（α，β）诱导产生抗病毒蛋白；EV71可在I型干扰素（α，β）诱导产生抗病毒蛋白的HeLa细胞中有效增殖。Objective To investigate human enterovirus 71 （ EV71 ） resistance to type I interferon induced antiviral effect. Methods After type I interferons （α,β） were incubated with HeLa cells, recombinant type I herpes simple virus （HSV-1） with green fluorescent protein （GFP） was inoculated onto the HeLa cells. HSV-1 proliferation was observed by GFP expression and PCR. After EVT1 was inoculated onto HeLa cells incubated with the same quantity of interferon, proliferation of EV71 were detected by RT- PCR of 2A gene. Results Recombinant HSV-1 GFP expression and viral DNA replication obviously decreased in HeLa cells incubated with type I interferon （α,β）. However, EV71 effectively proliferated in the interferon irritated HeLa cell by RT-PCR. Conclusion HcLa cell irritated by type I interferon （α,β） produced antiviral substance that inhibits HSV-1 proliferation. EVT1 resisted the antiviral substance induced by type I interferon and could significantly replicate in the HeLa cells.

关 键 词：肠道病毒属 干扰素I型 疱疹病毒I型 人 

分 类 号：R3[医药卫生—基础医学]