甲型肝炎病毒IgM AlphaLISA检测方法的初步建立  

Primary establishment of an alphaLISA assay for detection of HAV IgM

作　　者：甘星[1] 王颖[2] 宋娟[2] 崔雨[2] 孙鹏[2] 高晨[2] 李朝品[1] 韩俊[2] 

机构地区：[1]皖南医学院病原微生物学教研室,安徽241000 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室

出　　处：《中华实验和临床病毒学杂志》2012年第2期139-141,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：传染病重大专项[2009ZX10004-101,2008ZX10004-002];传染病重点实验室发展基金[2008SKLID102,2011SKLID104]

摘　　要：目的建立甲型肝炎病毒抗体IgM的AlphaLISA检测。方法浓缩甲型肝炎病毒抗原并生物素化，通过dot—Blot法摸索出生物素化的甲型肝炎病毒的最适量为6×10-8ng。优化供体珠、受体微珠、血清等实验反应条件，建立了甲型肝炎病毒的AlphaLISA的IgM检测方法。并对23份感染甲型肝炎病毒的IgM阳性患者和70份健康人的血清进行检测。结果AlphaLISA甲型肝炎病毒的IgM方法检测：灵敏度（真阳性率）为78％；特异度（真阴性率）为98．5％；假阳性率为1％；假阴性率为22％；阳性预测值为95％；阴性预测值为93％；准确度为94％。结论建立了均相、快速检测AlphaLISA甲型肝炎病毒IgM检测方法并得到初步应用。Objective To develop an AlphaLISA method for detection of antibody of Hepatitis A virus. Methods After hepatitis A virus antigen was concentrated and biotinylated, optimal biotinylated HAV antigen, donor bead, aceeptor bead concentration have been explored and determined by both dot- blotting and AlphaLISA methods. 97 samples including 23 serums from patients HAV infected and 70 serums from blood donors have been detected by new AIphaLISA method. Results The sensitivity and specificity for anti-HAV IgM were 78% and 98.5% , the positive and negative predictive value were 95% and 93%. Conclusion A homogeneous and fast AlphaLISA assay for detection of HAV IgM has been established by preliminary verification on samples.

关 键 词：肝炎病毒 甲型 免疫球蛋白M 监测 免疫学 

分 类 号：R4[医药卫生—临床医学] R51