甲状旁腺激素对去卵巢SD大鼠及其成骨细胞基质GLA蛋白表达的影响  

The effect of parathyroid hormone on expression of matrix GLA protein in ovariectomized SD rats and primary osteoblasts

作　　者：赖晓阳[1] 陈雪英[2] 方向南[1] 张美英[1] 姜醒华[2] 王平芳[3] 廖二元[3] 杨雅[1] 

机构地区：[1]南昌大学第二附属医院内分泌代谢科,330066 [2]南昌大学第二附属医院心血管内科,330066 [3]中南大学湘雅二医院代谢内分泌研究所

出　　处：《中华内分泌代谢杂志》2012年第4期330-334,共5页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（30860112）

摘　　要：目的观察甲状旁腺激素对去卵巢SD大鼠及其原代成骨细胞基质GLA蛋白（MGP）表达的影响，探讨MGP在绝经后骨质疏松症发病机制中的可能作用。方法36只SD雌鼠被分为3组，每组12只：假手术组、单纯去卵巢组（OVX组）和去卵巢加甲状旁腺激素干预组（PTH组）。PTH组大鼠去卵巢3周后，予甲状旁腺激素（2μg／kg，每周3次，持续12周）。所有大鼠18周后处死。每3周收集尿及血液。观察腰椎组织及骨密度变化，酶联免疫吸附（ELISA）法检测血清及尿液中MGP的量；免疫组化法观察腰椎未羧化MGP的表达，荧光实时定量观察腰椎及成骨细胞MGPmRNA的表达。结果甲状旁腺激素干预后去卵巢sD大鼠血清及尿液中MGP量，腰椎未羧化MGP表达、MGPmRNA表达与单纯去卵巢组比较差异有显著性（P〈0．05），甲状旁腺激素（1-34）在10-7mol／L、10-8mol／L、10-9mol／L上调成骨细胞MGPmRNA的表达较空白对照组分别为6．78倍、5．31倍和2．23倍，呈剂量依赖性，差异均有统计学意义（P〈0．05）。结论甲状旁腺激素对MGP基因及蛋白表达的影响可能在绝经后骨质疏松发病机制中发挥重要作用。Objective To observe the effect of parathyroid hormone on expression of matrix GLA protein （MGP） in ovariectomized SD rats and primary osteoblast, and to study the role of MGP on the possible mechanism of postmenopausal osteoporosis. Methods Thirty-six Sprague-Dawley female rats were allocated into 3 groups, 12 in each： sham operation group, ovariectomized group（ OVX group） , ovariectomized and parathyroid hormone treatment group. Animals in the parathyroid hormone group were injected parathyroid hormone （20 μg/kg, three times a week for 12 weeks） three weeks after ovarieetomy. All rats were sacrificed after 18 weeks. Urine and serum were collected every three weeks. Lumbar vertebral bones were observed by immunohistochemistry. Bone mineral density （BMD） of lumbar vertebra of rats was determined. The content of MGP in serum and urine was determined by enzyme-linked immunosorbent assay （ ELISA ）. Expression of undercarboxylated Matrix GLA Protein （ ucMGP ） was detected by immunochistochemistry. Relative quantification of MGP mRNA expression in lumbar vertebra bone was detected by Fluorescent real-time quantitative polymerause chain reaction. Results （ 1 ） 18 weeks after ovarieetomy, BMD of lumbar vertebra in OVX group was lower than those in sham group and parathyroid hormone group significantly （ P〈 0.05 ）. （2） The content of MGP in serum and urine was dynamic variation after treatment by parathyroid hormone, and it was significant compared with OVX group （ P〈0.05 ）. （ 3 ） Immunohistochemical localization of ucMGP was seen in lumbar vertebra in OVX group. （ 4 ） Relative quantification of MGP mRNA expression in lumbar vertebra in OVX group was increased significantly compared with other groups （ P〈0.01 ）. （ 5 ） parathyroid hormone （ 1-34 ） in 10-7mol/L, 10-8mol/L, 10-9 mol/L up-regulated MGP mRNA expression in primary osteoblasts about 6.78, 5.31, and 2.23 times than control respectively. It was in a dose-dependent manner. Conclusion The effect o

关 键 词：甲状旁腺激素 去卵巢大鼠 基质GLA蛋白 绝经后骨质疏松 

分 类 号：R580[医药卫生—内分泌]