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机构地区:[1]重庆医科大学附属第一医院老年科心血管病组,重庆400016 [2]重庆医科大学附属第一医院实验研究中心,重庆400016 [3]重庆医科大学附属第一医院超声心动图室,重庆400016 [4]重庆市西郊医院功能检查科,重庆400050
出 处:《基础医学与临床》2012年第5期539-543,共5页Basic and Clinical Medicine
基 金:重庆市卫生局医学科学技术研究项目(2009-2-290;2004-2-154);重庆市科委自然科学基金(CSTC;2007BB5276)
摘 要:目的观察大鼠梗死灶周围心肌C3G蛋白表达及异丙肾上腺素(ISO)对其的影响。方法建立雄性SD大鼠心肌梗死及假手术模型。将术后7 d仍存活的动物分为:1)心肌梗死组,2)假手术组,3)心肌梗死ISO组,4)假手术ISO组。分别腹腔注射给予1)及2)组0.9%氯化钠注射液5 mL/kg,3)及4)组分别给予ISO 5 mg/kg,每3天1次,连续12周。免疫印迹检测梗死灶周围心肌C3G蛋白的表达。结果干预后12周,梗死灶周围心肌C3G蛋白相对表达水平分别为:心肌梗死组(1.14±0.29,n=8),假手术组(0.90±0.10,n=6),心肌梗死ISO组(1.51±0.18,n=10),假手术ISO组(0.97±0.26,n=8)。心肌梗死组较假手术组、心肌梗死ISO组较假手术ISO组及心肌梗死ISO组较心肌梗死组梗死灶周围心肌C3G蛋白的表达均显著增加(P<0.05)。结论梗死灶周围心肌C3G蛋白表达显著增加,而ISO处理进一步使表达显著增加。Objective C3G(CrkSH3-binding guanine nucleotide exchange factor) protein expression was investigated in myocardium around the infarcted zones in rats with ISO treatment or not.Methods Myocardial infarction(MI) and sham-operation models were established.Seven days after operation,surviving rats were divided into MI,sham,MI+ISO and sham+ISO groups respectively,and were treated with physiological buffered saline 5 mL/kg or ISO 5 mg/kg intraperitoneally once every 3 days for 12 weeks respectively.C3G protein expression in myocardium around the infarcted zones was detected by Western blot.Results Twelve weeks after treatment,C3G protein expression was(1.14±0.29,n=8) in the MI group,(0.90±0.10,n=6) in the sham group,(1.51±0.18,n=10) in the MI+ISO group and(0.97±0.26,n=8) in the sham+ISO group respectively.The C3G protein expression in myocardium around the infarcted zones was significantly increased in the MI group compared with the sham group,and in the MI+ISO group compared with the sham+ISO group,and in the MI+ISO groupcompared with the MI group respectively(All P0.05).Conclusions C3G protein expression significantly increased in myocardium around the infarcted zones.ISO treatment can further increase its expression in myocardium around the infarcted zones.
关 键 词:鸟嘌呤核苷交换因子 C3G 心肌梗死 异丙肾上腺素
分 类 号:R542.22[医药卫生—心血管疾病]
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