青蒿琥酯诱导食管癌Eca109细胞凋亡  

Inhibition of esophageal cancer cell Eca109 by Artesunate through induction of cell apoptosis

作　　者：刘亮[1] 左静[2] 左连富[1] 郭建文[1] 

机构地区：[1]河北医科大学第四医院肿瘤研究所流式细胞室,河北石家庄050011 [2]河北医科大学第四医院肿瘤内科,河北石家庄050011

出　　处：《基础医学与临床》2012年第5期548-553,共6页Basic and Clinical Medicine

基　　金：河北省普通高等学校强势特色学科肿瘤学建设经费[冀教高(2005)52号];河北省医学科学研究重点课题(20110131)

摘　　要：目的研究青蒿琥酯诱导食管癌Eca109细胞凋亡作用。方法不同浓度的青蒿琥酯(0、10、20、40 mg/L)作用Eca109细胞24 h,流式细胞术方法检测细胞凋亡、周期及细胞中BCL-2和BAX蛋白的表达水平。Western blot检测细胞中BCL-2及BAX蛋白表达水平。结果 102、04、0 mg/L青蒿琥酯作用Eca109细胞24 h后细胞凋亡率分别为5.26%±0.20%、18.39%±1.58%、26.78%±1.09%,与对照组(1.52%±0.09%)相比显著增高(P<0.05),且具有剂量依赖性。流式细胞术检测显示,10、20、40 mg/L青蒿琥酯组Eca109细胞中BCL-2蛋白表达水平分别为369.99±16.22、322.34±14.111、79.01±10.35,与对照组(420.28±6.04)相比显著降低(P<0.05),细胞增殖指数也显著降低(P<0.05)。10、20、40 mg/L青蒿琥酯组Eca109细胞中BAX蛋白表达水平分别为292.65±9.47、422.23±7.46、479.22±8.33,与对照组(248.95±5.47)相比显著增高(P<0.05)。Western blot检测细胞中BCL-2及BAX蛋白表达水平同流式细胞术检测结果一致。结论青蒿琥酯通过调节Eca109细胞中BCL-2和BAX蛋白表达水平和细胞增殖,而诱导Eca109细胞凋亡。Objective To research the apoptosis-inducing function of artesunate on esophageal cancer cells Eca109.Methods Cell apoptosis,cell cycle,BCL-2 and BAX protein expression level of Eca109 cells after the treatment of various concentrations of artesunate（Art）（0,10,20,40 μg/mL） for 24 h were detected by flow cytometry（FCM）.BCL-2 and BAX protein expression level of Eca109 cells were also detected by Western blot.Results Cell apoptosis rate of Eca109 cells after the treatment of different concentrations of Art（10,20,40 mg/L） for 24 h was significantly higher than that of control group（1.52%±0.09%）（P0.05）,in a concentration-dependent manner.The BCL-2 protein expression level in Eca109 cells of Art（10,20,40 mg/L） groups was significantly lower than that of control group.The proliferation index of Art groups was also significantly lower than that of control group.The BAX protein expression level in Eca109 cells of Art（10,20,40 mg/L） groups was significantly higher than that of control group.The results of BCL-2 and BAX protein expression level detected by Western blot were consistent with flow cytometry results.Conclusion Artesunate can induce cell apoptosis of Eca109 cells by regulating the BCL-2,BAX protein expression level and cell proliferation.

关 键 词：流式细胞术 食管癌 青蒿琥酯 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R735.1[医药卫生—中医学]