益骨汤含药血清对新生大鼠成骨细胞增殖及分泌IL-6的影响  

Effect of YIGUTANG Containing Serum on Osteoblast Proliferation and IL-6 Secretion

作　　者：陈芳[1] 朱以良[1] 杨李[1] 唐瑛[2] 

机构地区：[1]广州军区武汉总医院医学实验科,武汉430070 [2]海军医学研究所,上海200433

出　　处：《实验动物与比较医学》2012年第2期146-148,共3页Laboratory Animal and Comparative Medicine

基　　金：广州军区武汉总医院课题

摘　　要：目的探讨益骨汤含药血清对新生大鼠成骨细胞增殖及分泌IL-6的影响。方法采用酶消化法获得新生大鼠的成骨细胞，然后以益骨汤含药血清作用于成骨细胞，用MTT法检测成骨细胞增殖，ELISA法测定培养上清中IL-6的含量。结果益骨汤各剂量组（A、B、C组）能促进成骨细胞的增殖，能使其分泌IL-6的能力下降，与空白对照组（E组）比较有显著性差异（P〈0．05）。结论益骨汤含药血清能够促进成骨细胞的增殖，同时抑制成骨细胞分泌IL-6。Objective To research the effect of YIGUTANG containing serum on osteoblast proliferation which was from the skull of new born Wistar rat in vitro, and IL-6 secretion. Methods Iliac bone specimens were obtained from the newborn rat calvaria, after that the bone pieces were digested by collagenase-trypsin. The proliferation of osteoblast was observed using MTT reduction assay. The IL-6 in the supernatant was detected by ELISA. Results The proliferation of osteoblast could be stimulated by the serum with different dosages of absorbed YIGUTANG （group A, B , C）. Meanwhile, the levels of IL-6 secretion in group A, B and C could be significantly decreased compared with the control group （P〈0.05）. Conclusion The proliferation of osteoblast can be stimulated by the serum with absorbed YIGUTANG and the capability of producing IL-6 in osteoblast also can be restrained by the serum.

关 键 词：益骨汤含药血清 大鼠成骨细胞 细胞增殖 白介素-6(IL-6) 

分 类 号：R285.5[医药卫生—中药学]