湖北河岸带植物中华蚊母树遗传多样性的SRAP分析  

SRAP Analysis of Genetic Diversity of Riparian Plant Distylium chinense in Hubei Province

作　　者：谢春花[1] 李晓玲[1] 栾春艳[1] 杨进[1] 陈发菊[1] 李争艳 

机构地区：[1]三峡大学生物技术研究中心,天然产物研究与利用湖北省重点实验室,宜昌443002 [2]宜昌三峡植物园,宜昌443111

出　　处：《植物研究》2012年第3期311-319,共9页Bulletin of Botanical Research

基　　金：宜昌市科技计划发展项目(A2007103-03)

摘　　要：利用SRAP分子标记技术,对湖北省河岸带植物中华蚊母树的4个自然居群和1个迁地居群的遗传多样性和遗传结构进行了分析。结果表明,中华蚊母树物种具有较高水平的遗传多样性,7对SRAP引物进行PCR扩增的多态性位点百分率(PPF)为80.43%,每个位点的等位基因数(A)为2,有效等位基因数(Ae)为1.34,总遗传多样性Nei’s基因多样性指数(Hp)为0.215 9,Shannon信息多样性指数(I)为0.350 9。在居群水平上,5个居群总的遗传变异Ht为0.218 8,居群内的遗传变异Hs为0.193 4,居群间的遗传分化系数Gst为0.116 1,表明在总的遗传变异中有88.39%变异存在于居群内,仅11.61%存在于居群间,居群间的基因流Nm为3.807 2,表明居群间有较大程度的基因交流。UPGMA聚类分析和主成分分析显示中华蚊母树主要分为两个居群组,在长江三峡沿岸香溪和乐天溪由于遗传距离比较近聚为一小类再与高家堰聚为一大类,而沿渡河和三峡植物园居群聚为另一大类,表明迁地居群三峡植物园的中华蚊母树与来自巴东沿渡河居群的样本亲缘关系最近,且三峡植物园迁地保护居群基本保育了其遗传多样性总水平。同时在分析讨论了中华蚊母树遗传多样性与其繁育系统、生境及其起源进化的关系的基础上,评价了中华蚊母树的保护策略,并在评价保护成果的基础上,提出了今后进一步保育的策略。结果还表明SRAP标记是分析中华蚊母树遗传多样性和遗传结构非常可靠的一种标记,而且这是使用SRAP标记研究中华蚊母树的首次报道。The genetic diversity and the genetic structure of four in situ populations and one ex situ conservation population of riparian plant Distylium chinense in Hubei province was investigated using sequence-related ampli- fied polymorphism （SRAP） molecular marker technique. A high level of genetic diversity at the species level was observed with the selected 7 SRAP primer combinations generating 46 discernible DNA fragments, of which 37 （80.43%） were polymorphic. The observed number of alleles （A）, the mean effective number of alleles （Ao）, Nei＇s gene diversity index （Hp） and Shannon＇ s information index （I） was 2.00, 1.34, 0. 215 9 and 0. 350 9, respectively. Within populations, the genetic variation was found to be mainly caused by the variation within populations, accounting for 88.39% of the total genetic variation and only 11.61% among the popula- tions. The gene flow of D. chinense between populations was 3. 807 2, which was a relative high gene flow and could efficiently prevent gene drift and maintain current genetic structure. Based on unweighted pair-group meth- od with arithmetic averages （UPGMA） dendrogram and principal coordinates analysis （PCA） diagram populations studied may be divided into two groups. Most accessions along riversides in Hubei province Letianxi, Xiangxi and Gaojiayan populations were found in one group, which was further divided into , the five including two subgroups that corresponded to the their geographic distance. Yanduhe and Three Gorges Botanical Garden popula- tions were clustered into another group, showing that the accessions of ex situ conservation population had rela- tively high relatedness with those from Yanduhe population. The mean genetic diversity in ex situ conservation population was higher than those in situ populations. Hence, the ex situ conservation program of Three Gorges Botanical Garden was considered to be successful in preserving genetic diversity of D. chinense. Moreover, com- bining the analysis of the genetic diversity

关 键 词：中华蚊母树 SRAP 遗传多样性 遗传结构 保护策略 

分 类 号：S727.22[农业科学—林木遗传育种]