正交试验法优选烟草中辅酶Q_(10)的匀浆提取工艺  

Optimization of Homogenate Extraction Technology of CoQ_(10) in Tobacco Leaves by Orthogonal Experiments

作　　者：李春英[1] 赵春建[1] 张宝友[1] 刘帅华[1] 刘德曼[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2012年第3期373-376,共4页Bulletin of Botanical Research

基　　金：黑龙江省自然科学基金项目(C201024);中央高校基本科研业务费专项资金项目(DL10BA05);黑龙江省政府博士后科研启动金

摘　　要：以烟草叶片为原料,用正交试验法优化了烟草中辅酶Q10的匀浆提取工艺,考察了匀浆时间、乙醇体积分数、液料比和提取次数4个因素对辅酶Q10提取率的影响,确立了烟草中辅酶Q10的优化匀浆提取工艺条件为:提取溶剂为85%乙醇,匀浆时间6 min,液料比9∶1(mL/g),提取次数2次。将该法与索氏提取进行了对比。结果表明,匀浆提取烟草中辅酶Q10的提取率为12.82μg·g-1,与索氏提取的提取率相当,但匀浆提取所用提取时间短,提取溶剂用量少,因此匀浆法具有明显的优势。Using tobacco leaves as materials, the homogenate extraction technology of coenzyme Qlo （CoQ10） in mized homogenate extraction technology was determined as follows： 85% ethanol as extraction solvent, homoge- nate time 6 min,ratio of liquid to material 9：l（mL/g）, 2 extraction cycles. A comparison between homogenate and Soxhlet extractions was conducted. The extraction yield of CoQ10 was 12.82 μg g-1 by homogenate extraction, which was almost equivalent to Soxhlet extraction. Homogenate extraction is faster and needs less solvent, thereby homogenate extraction has an advantage over Soxhlet extraction.

关 键 词：烟草 辅酶Q10 匀浆提取 正交试验 

分 类 号：Q949.9[生物学—植物学]