细胞固定化方法制备微藻光电极的研究  

Characterization of the Photoelectrode Based on the Immobilization of Microalgae

作　　者：吕艳霞[1,2] 陈兆安[1] 陆洪斌[1] 邓麦村[1] 薛松[1] 张卫[1] 

机构地区：[1]中国科学院大连化学物理研究所海洋生物产品工程组,大连116023 [2]中国科学院研究生院,北京100039

出　　处：《中国生物工程杂志》2012年第4期96-102,共7页China Biotechnology

基　　金：国家"973"计划(2009CB220004);国家自然科学基金(20806081);中科院知识创新工程重要方向(KSCX2-YW-G-073;KSCX2-YW-373-2;KGCX2-YW-223)资助项目

摘　　要：通过将微藻细胞固定在平面多孔碳纸上,制备微藻光电极,并在三电极体系电解液中加入电子介体进行测试,可产生与光照同步的光电流响应。考察了不同固定化方法、不同微藻及不同电子介体的光电流响应,结果表明硅溶胶-凝胶法制备的光电极光电流响应最佳,且对于亚心形四爿藻、金藻、莱茵衣藻、蛋白核小球藻、聚球藻等5种微藻都适用,表明该制备方法对不同微藻具有较好的通用性。电子介体的研究表明苯醌及其衍生物由于氧还电位较高,具有较好的阳极光电流响应特性,而甲基紫精氧还电位较低,具有较好的阴极光电流响应。The microalgae photoelectrode prepared by immobilizing microalgae on the porous carbon paper displayed light-dependent electrogenic activity with electron mediators in a three-electrode system.The effect of different immobilization methods,diverse genera of microalgae and different electron mediators on photocurrent responses were investigated.The results showed that microalgae immobilized on the anode via silica sol-gel encapsulation exhibited the best photocurrent response.The diverse genera of microalgae including eukaryotic alga（Tetraselmis subcordiformis,Chlorella pyrenoidosa,Chlamydomonas reinhardtii CC 124,Isochrysis zhanjiangensis） and prokaryon alga（Synechococcus sp.PCC 7942） had similar response,demonstrating that the electrons from photosynthetic electron transfer chain of diverse genera of microalgae could be transferred to the electrode via exogenous artificial electron mediator.Benzoquinone and its derivatives as the electron mediator had anodic photocurrent response due to high redox potential,while methyl viologen exhibited low cathodic photocurrent response because of its low redox potential.

关 键 词：微藻 硅溶胶-凝胶 固定化 光合电子传递 

分 类 号：Q819[生物学—生物工程]